Anti-EEF2/Elongation factor 2 (phospho T56) antibody (ab53114)
Key features and details
- Rabbit polyclonal to EEF2/Elongation factor 2 (phospho T56)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-EEF2/Elongation factor 2 (phospho T56) antibody
See all EEF2/Elongation factor 2 primary antibodies -
Description
Rabbit polyclonal to EEF2/Elongation factor 2 (phospho T56) -
Host species
Rabbit -
Specificity
ab53114 detects endogenous levels of EEF2/Elongation factor 2 only when phosphorylated at threonine 56.
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Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Mouse
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Immunogen
Synthetic peptide corresponding to Human EEF2/Elongation factor 2.
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General notes
This product was previously labelled as EEF2
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab53114 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-EEF2/Elongation factor 2 (phospho T56) antibody (ab53114)All lanes : Anti-EEF2/Elongation factor 2 (phospho T56) antibody (ab53114) at 1/300 dilution
Lane 1 : Extract from NIH 3T3 cells
treated with serum (10%, 30mins) with Immunising peptide
Lane 2 : Extract from NIH 3T3 cells
treated with serum (10%, 30mins)
Predicted band size: 95 kDa
Observed band size: 95 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2/Elongation factor 2 (phospho T56) antibody (ab53114)ab53114 at 1/50 dilution staining EEF2/Elongation factor 2 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.
