Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Lin28A antibody [EPR4640] - BSA and Azide free (ab226089)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4640] to Lin28A - BSA and Azide free
  • Suitable for: IP, IHC-P, Flow Cyt, ICC/IF, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Lin28A antibody [EPR4640] - BSA and Azide free
    See all Lin28A primary antibodies

  • Description

    Rabbit monoclonal [EPR4640] to Lin28A - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-P, Flow Cyt, ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab226089 is the carrier-free version of ab124765 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab226089 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse and Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR4640

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28A antibody [EPR4640] - BSA and Azide free (ab226089)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28A antibody [EPR4640] - BSA and Azide free (ab226089)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling Lin28A with purified ab124765 at 1/50 dilution (12 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124765).

  • Immunocytochemistry/ Immunofluorescence - Anti-Lin28A antibody [EPR4640] - BSA and Azide free (ab226089)
    Immunocytochemistry/ Immunofluorescence - Anti-Lin28A antibody [EPR4640] - BSA and Azide free (ab226089)

    Immunocytochemistry/ Immunofluorescence analysis of JAR (Human placenta choriocarcinoma epithelial cell) cells labeling Lin28A with purified ab124765 at 1/500 dilution (1.2 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124765).

  • Flow Cytometry - Anti-Lin28A antibody [EPR4640] - BSA and Azide free (ab226089)
    Flow Cytometry - Anti-Lin28A antibody [EPR4640] - BSA and Azide free (ab226089)

    Flow Cytometry analysis of NCCIT (Human pluripotent embryonic carcinoma epithelial cell) cells labeling Lin28A with purified ab124765 at 1/60 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124765).

  • Immunoprecipitation - Anti-Lin28A antibody [EPR4640] - BSA and Azide free (ab226089)
    Immunoprecipitation - Anti-Lin28A antibody [EPR4640] - BSA and Azide free (ab226089)

    ab124765 (purified) at 1/30 dilution (2ug) immunoprecipitating Lin28A in NCCIT whole cell lysate. NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate 10ug
    Lane 2 (+): ab124765 & NCCIT whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124765 in NCCIT whole cell lysate

    For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/1000 dilution.

    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124765).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28A antibody [EPR4640] - BSA and Azide free (ab226089)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28A antibody [EPR4640] - BSA and Azide free (ab226089)

    ab124765 (unpurified), at 1/100 dilution, staining Lin28A in paraffin-embedded Human seminoma tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124765).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Lin28A antibody [EPR4640] - BSA and Azide free (ab226089)
    Immunocytochemistry/ Immunofluorescence - Anti-Lin28A antibody [EPR4640] - BSA and Azide free (ab226089)

    ab124765 (unpurified), at 1/100 dilution, staining Lin28A in NCCIT cells by Immunofluorescence.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124765).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28A antibody [EPR4640] - BSA and Azide free (ab226089)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28A antibody [EPR4640] - BSA and Azide free (ab226089)

    ab124765 (unpurified) showing positive staining in Glioma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124765).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28A antibody [EPR4640] - BSA and Azide free (ab226089)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28A antibody [EPR4640] - BSA and Azide free (ab226089)

    ab124765 (unpurified) showing positive staining in Dysgerminoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124765).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28A antibody [EPR4640] - BSA and Azide free (ab226089)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28A antibody [EPR4640] - BSA and Azide free (ab226089)

    ab124765 (unpurified) showing positive staining in Embryonal carcinoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124765).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28A antibody [EPR4640] - BSA and Azide free (ab226089)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28A antibody [EPR4640] - BSA and Azide free (ab226089)

    ab124765 (unpurified) showing negative staining in Normal brain tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124765).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Anti-Lin28A antibody [EPR4640] - BSA and Azide free (ab226089)
    Anti-Lin28A antibody [EPR4640] - BSA and Azide free (ab226089)

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