Antibodies, Proteins, Kits and Reagents for Life Science

318 288 ₸ Product size

100 µl 318 288 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Dynamin 1 antibody [EP801Y] (ab52611)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP801Y] to Dynamin 1
Suitable for: ICC/IF, WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-Dynamin 1 antibody [EP801Y]
See all Dynamin 1 primary antibodies
Description

Rabbit monoclonal [EP801Y] to Dynamin 1
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Mouse
IHC-P	Mouse Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide within Human Dynamin 1 aa 800-900 (C terminal). The exact sequence is proprietary.
Positive control
- 3T3 cell lysate, human brain tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP801Y
Isotype

IgG
Research areas

Western blot - Anti-Dynamin 1 antibody [EP801Y] (ab52611)

All lanes : Anti-Dynamin 1 antibody [EP801Y] (ab52611) at 1/2000 dilution (purified)

Lane 1 : C6 cell lysate
Lane 2 : NIH/3T3 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 97 kDa
Observed band size: 97 kDa

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-Dynamin 1 antibody [EP801Y] (ab52611)

Anti-Dynamin 1 antibody [EP801Y] (ab52611) at 1/10000 dilution (purified) + SH-SY5Y cell lysate at 10 µg

Secondary
HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

Predicted band size: 97 kDa
Observed band size: 97 kDa

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence - Anti-Dynamin 1 antibody [EP801Y] (ab52611)

Immunofluorescence staining of Neuro-2a cells with purified ab52611 at a working dilution of 1/300, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab52611 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 1 antibody [EP801Y] (ab52611)

Immunohistochemical staining of paraffin embedded mouse cerebral cortex with purified ab52611 at a working dilution of 1/50. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 1 antibody [EP801Y] (ab52611)

Immunohistochemical staining of paraffin embedded human cerebral cortex with purified ab52611 at a working dilution of 1/50. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Western blot - Anti-Dynamin 1 antibody [EP801Y] (ab52611)

Anti-Dynamin 1 antibody [EP801Y] (ab52611) at 1/2000 dilution (unpurified) + 3T3 cell lysate at 10 µg

Secondary
Goat anti-rabbit HRP antibody at 1/2000 dilution

Predicted band size: 97 kDa
Observed band size: 97 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 1 antibody [EP801Y] (ab52611)

Unpurified ab52611 at 1/100-1/250 dilution staining human brain tissue; paraffin embedded.
Anti-Dynamin 1 antibody [EP801Y] (ab52611)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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