Anti-Dynamin 1 antibody (ab150055)
Key features and details
- Rabbit polyclonal to Dynamin 1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Dynamin 1 antibody
See all Dynamin 1 primary antibodies -
Description
Rabbit polyclonal to Dynamin 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide corresponding to Human Dynamin 1 aa 600-700 conjugated to keyhole limpet haemocyanin.
(Peptide available asab139745) -
Positive control
- This antibody gave a positive signal in Human, Mouse and Rat Brain tissue lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150055 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseAll applications HorseCowDogPigChimpanzeeMacaque monkeyApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 102 kDa (predicted molecular weight: 97 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 102 kDa (predicted molecular weight: 97 kDa).Target
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Function
Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes. Involved in receptor-mediated endocytosis. -
Sequence similarities
Belongs to the dynamin family.
Contains 1 GED domain.
Contains 1 PH domain. -
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton. Microtubule-associated. - Information by UniProt
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Database links
- Entrez Gene: 1759 Human
- Entrez Gene: 13429 Mouse
- Entrez Gene: 140694 Rat
- Omim: 602377 Human
- SwissProt: Q05193 Human
- SwissProt: P39053 Mouse
- SwissProt: P21575 Rat
- Unigene: 522413 Human
see all -
Alternative names
- B dynamin antibody
- D100 antibody
- DNM 1 antibody
see all
Images
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Western blot - Anti-Dynamin 1 antibody (ab150055)All lanes : Anti-Dynamin 1 antibody (ab150055) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 102 kDa why is the actual band size different from the predicted?
Additional bands at: 70 kDa, 95 kDa, 99 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Datasheets and documents
References (1)
ab150055 has been referenced in 1 publication.
- Li J et al. Xanthan Gum Ameliorates Osteoarthritis and Mitigates Cartilage Degradation via Regulation of the Wnt3a/ß-Catenin Signaling Pathway. Med Sci Monit 25:7488-7498 (2019). PubMed: 31587011
Images
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Western blot - Anti-Dynamin 1 antibody (ab150055)All lanes : Anti-Dynamin 1 antibody (ab150055) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 102 kDa why is the actual band size different from the predicted?
Additional bands at: 70 kDa, 95 kDa, 99 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
