Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Pan-Farnesylation antibody [EPR19409] (ab199481)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19409] to Pan-Farnesylation
  • Suitable for: Dot blot, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Pan-Farnesylation antibody [EPR19409]
    See all Pan-Farnesylation primary antibodies

  • Description

    Rabbit monoclonal [EPR19409] to Pan-Farnesylation

  • Host species

    Rabbit

  • Specificity

    The antibody was developed specifically for farnesylated LKB1. Refer to the literature (PMID: 24295069), it might also interact with other farnesylated proteins.

  • Tested applications

    Suitable for: Dot blot, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HA-LKB1(WT) transfected 293T lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19409

  • Isotype

    IgG

Images

  • Dot Blot - Anti-Pan-Farnesylation antibody [EPR19409] (ab199481)
    Dot Blot - Anti-Pan-Farnesylation antibody [EPR19409] (ab199481)

    Dot blot analysis of Pan-Farnesylation labeled with ab199481 at 1/1000 dilution.

    Lane 1: LKB1 farnesylated peptide;

    Lane 2: LKB1 non-farnesylated peptide.

    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution was used as secondary antibody.

    Blocking and diluting buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Western blot - Anti-Pan-Farnesylation antibody [EPR19409] (ab199481)
    Western blot - Anti-Pan-Farnesylation antibody [EPR19409] (ab199481)
    All lanes : Anti-Pan-Farnesylation antibody [EPR19409] (ab199481) at 1/1000 dilution

    Lane 1 : HA-LKB1(WT) transfected 293T (Human epithelial cell line from embryonic kidney) lysate
    Lane 2 : HA-LKB1(C433S) transfected 293T (Human epithelial cell line from embryonic kidney) lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 49 kDa


    Exposure time: 2 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The lysates were kindly provided by the collaborator (Dr. Sheng-Cai Lin, Xiamen University). Based on the sequence alignment, this product may recognize human, mouse and rat species. The ab was developed specifically for farnesylated LKB1. Refer to the literature (PMID: 24295069), it might also interact with other farnesylated proteins.

  • Anti-Pan-Farnesylation antibody [EPR19409] (ab199481)
    Anti-Pan-Farnesylation antibody [EPR19409] (ab199481)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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