Anti-Lysophospholipase 1/LPL-I antibody [EPR3666] - BSA and Azide free (ab247525)
![Anti-Lysophospholipase 1/LPL-I antibody [EPR3666] - BSA and Azide free (ab247525)](https://www.abcam.com/ps/products/247/ab247525/Images/ab247525-403258-anti-lysophospholipase-1-lpl-i-antibody-epr3666-bsa-and-azide-free-western-blot.jpg "Anti-Lysophospholipase 1/LPL-I antibody [EPR3666] - BSA and Azide free (ab247525)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3666] to Lysophospholipase 1/LPL-I - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Lysophospholipase 1/LPL-I antibody [EPR3666] - BSA and Azide free
See all Lysophospholipase 1/LPL-I primary antibodies -
Description
Rabbit monoclonal [EPR3666] to Lysophospholipase 1/LPL-I - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab247525 is the carrier-free version of ab91600. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab247525 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR3666 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3666] - BSA and Azide free (ab247525)
This data was developed using ab91600, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 whole cell lysate (40 µg)
Lane 2: LYPLA1 knockout HAP1 whole cell lysate (40 µg)
Lane 3: Mouse spleen whole cell lysate (40 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab91600 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab91600 was shown to specifically react with LYPLA1 in wild-type HAP1 cells. No band was observed when LYPLA1 knockout samples were examined. Wild-type and LYPLA1 knockout samples were subjected to SDS-PAGE. ab91600 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with ab216773, Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed and ab216776, Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3666] - BSA and Azide free (ab247525)](https://www.abcam.com/ps/products/247/ab247525/Images/ab247525-397981-anti-lysophospholipase-1-lpl-i-antibody-epr3666-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3666] - BSA and Azide free (ab247525)All lanes : Anti-Lysophospholipase 1/LPL-I antibody [EPR3666] (ab91600) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-rabbit HRP at 1/2000 dilution
Predicted band size: 25 kDaThis data was developed using ab91600, the same antibody clone in a different buffer formulation.
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![Anti-Lysophospholipase 1/LPL-I antibody [EPR3666] - BSA and Azide free (ab247525)](https://www.abcam.com/ps/products/247/ab247525/Images/ab247525-3-benefits-of-recombinant-antibodies.png)
Anti-Lysophospholipase 1/LPL-I antibody [EPR3666] - BSA and Azide free (ab247525)