Anti-CerS2 antibody [OTI3D9] (ab279372)
![Anti-CerS2 antibody [OTI3D9] (ab279372)](https://www.abcam.com/ps/products/279/ab279372/Images/ab279372-5-AntiCerS2-antibody-OTI3D9-immunhistochemistry-Carcinoma-lung-tissue-human.jpg "Anti-CerS2 antibody [OTI3D9] (ab279372)")
Key features and details
- Mouse monoclonal [OTI3D9] to CerS2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
Overview
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Product name
Anti-CerS2 antibody [OTI3D9]
See all CerS2 primary antibodies -
Description
Mouse monoclonal [OTI3D9] to CerS2 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human CerS2 aa 62-136. NP_859530
Database link: Q96G23 -
Positive control
- WB: Jurkat, K562, HepG2, A549, HeLa cells; HEK-293T cells. IHC-P: Carcinoma of human lung tissue, breast tissue,
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
OTI3D9 -
Isotype
IgG2a -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CerS2 antibody [OTI3D9] (ab279372)
Paraffin embedded carcinoma of human lung tissue stained for CERS2 using ab279372 at 1/1000 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer pH8.5 at 120°C for 3 minutes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CerS2 antibody [OTI3D9] (ab279372)](https://www.abcam.com/ps/products/279/ab279372/Images/ab279372-4-AntiCerS2-antibody-OTI3D9-immunhistochemistry-breast-tissue-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CerS2 antibody [OTI3D9] (ab279372)Paraffin embedded Human breast tissue stained for CERS2 using ab279372 at 1/1000 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer pH8.5 at 120°C for 3 minutes.
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![Western blot - Anti-CerS2 antibody [OTI3D9] (ab279372)](https://www.abcam.com/ps/products/279/ab279372/Images/ab279372-3-AntiCerS2-antibody-OTI3D9-westernblot-HEK293T-Cells-wildtype-and-knockout.jpg)
Western blot - Anti-CerS2 antibody [OTI3D9] (ab279372)Lane 1 : Anti-CerS2 antibody [OTI3D9] (ab279372) at 1/500 dilution
Lane 2 : Anti-CerS2 antibody [OTI3D9] (ab279372) at 1/500 dilution (anti-HSP90AB1)
Lanes 3-4 : anti-HSP90AB1 at 1/500 dilution
Lanes 1 & 3 : Wild-type HEK-293T cells
Lanes 2 & 4 : CERS2-Knockout HEK-293T cells
Lysates/proteins at 10 µg per lane.
Predicted band size: 45 kDaThe blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody for loading control purposes.
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![Western blot - Anti-CerS2 antibody [OTI3D9] (ab279372)](https://www.abcam.com/ps/products/279/ab279372/Images/ab279372-1-AntiCerS2-antibody-OTI3D9-westernblot-Jurkat-K562-HepG2-A549-HeLa-cells.jpg)
Western blot - Anti-CerS2 antibody [OTI3D9] (ab279372)All lanes : Anti-CerS2 antibody [OTI3D9] (ab279372) at 1/500 dilution
Lane 1 : Jurkat cell extract
Lane 2 : K562 cell extract
Lane 3 : HepG2 cell extract
Lane 4 : A549 cell extract
Lane 5 : HeLa cell extract
Lysates/proteins at 35 µg per lane.
Predicted band size: 45 kDa