Anti-KLF15 antibody (ab109426)
Key features and details
- Rabbit polyclonal to KLF15
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KLF15 antibody
See all KLF15 primary antibodies -
Description
Rabbit polyclonal to KLF15 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in both Human Liver and Heart tissue lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109426 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications ChimpanzeeMacaque monkeyGorillaOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). Abcam recommends using milk as the blocking agent.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). Abcam recommends using milk as the blocking agent.Target
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Function
Transcriptional activator. Binds to the GA element of the CLCNKA promoter. -
Tissue specificity
Highly expressed in liver, followed by heart, skeletal muscle, and kidney. Not expressed in bone marrow or lymphoid tissues. -
Sequence similarities
Belongs to the Sp1 C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 28999 Human
- GenBank: NP_054798.1 Human
- Omim: 606465 Human
- SwissProt: Q9UIH9 Human
- Unigene: 272215 Human
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Alternative names
- Kidney enriched Kruppel like factor antibody
- Kidney-enriched krueppel-like factor antibody
- KKLF antibody
see all
Images
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Western blot - Anti-KLF15 antibody (ab109426)All lanes : Anti-KLF15 antibody (ab109426) at 1 µg/ml (Milk Blocking (3%))
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Human heart tissue lysate - total protein (ab29431)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 44 kDa
Additional bands at: 21 kDa, 27 kDa, 35 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Datasheets and documents
References (0)
ab109426 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-KLF15 antibody (ab109426)All lanes : Anti-KLF15 antibody (ab109426) at 1 µg/ml (Milk Blocking (3%))
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Human heart tissue lysate - total protein (ab29431)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 44 kDa
Additional bands at: 21 kDa, 27 kDa, 35 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
