Human YKL-40 ELISA Kit (ab255719)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3.9 pg/ml
- Range: 23.44 pg/ml - 1500 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human, Rhesus monkey
Overview
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Product name
Human YKL-40 ELISA Kit
See all YKL-40/CHI3L1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 2.4% Inter-assay Sample n Mean SD CV% Serum 3 1.7% -
Sample type
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
3.9 pg/ml -
Range
23.44 pg/ml - 1500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 103 100% - 107% Urine 102 98% - 109% Serum 110 101% - 122% Hep Plasma 108 103% - 118% EDTA Plasma 118 113% - 124% Cit plasma 116 113% - 123% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human, Rhesus monkey
Does not react with: Cow -
Product overview
Human YKL-40 ELISA Kit (ab255719) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of YKL-40 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human YKL-40 with 3.9 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
YKL-40 (Chitinase-3-like protein 1) is a carbohydrate binding lectin with a preference for chitin. YKL-40 may play a role in tissue remodeling and cell response to changes in environment. It plays a the Th2 response of T-helper cells and IL-13-induced inflammation by regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation, and M2 macrophage differentiation. It regulated antibacterial response in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human YKL-40 Capture Antibody 1 x 600µl 10X Human YKL-40 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human YKL-40 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Carbohydrate-binding lectin with a preference for chitin. May play a role in defense against pathogens, or in tissue remodeling. May play an important role in the capacity of cells to respond to and cope with changes in their environment. -
Tissue specificity
Present in activated macrophages, articular chondrocytes, synovial cells as well as in liver. Undetectable in muscle tissues, lung, pancreas, mononuclear cells, or fibroblasts. -
Involvement in disease
A genetic variation in CHI3L1 is associated with susceptibility to asthma-related traits type 7 (ASRT7) [MIM:611960]. Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing and dyspnea, bronchial hyperresponsiveness (BHR) as assessed by methacholine challenge test, serum IgE levels, atopy, and atopic dermatitis. -
Sequence similarities
Belongs to the glycosyl hydrolase 18 family. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
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Alternative names
- 39 kDa synovial protein
- ASRT7
- Cartilage glycoprotein 39
see all -
Database links
- Entrez Gene: 1116 Human
- Omim: 601525 Human
- SwissProt: P36222 Human
- Unigene: 382202 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human YKL-40 standard curve comparisonStandard Curve comparison between human YKL-40 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of human YKL-40 standard curve in Sample Diluent NS.The YKL-40 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native YKL-40 in human serum and plasma samples.The concentrations of YKL-40 were measured in duplicates, interpolated from the YKL-40 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:40, plasma (citrate) 1:20, plasma (heparin) 1:20, and plasma (EDTA) 1:80. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean YKL-40 concentration was determined to be 37.59 ng/mL in neat serum, 16.17 ng/mL in neat plasma (citrate), 18.92 in neat plasma (heparin), and 67.92 ng/mL in neat plasma (EDTA).
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Interpolated concentrations of native YKL-40 in human urine and cell culture supernatant samples.The concentrations of YKL-40 were measured in duplicates, interpolated from the YKL-40 standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 100%, HepG2 cell culture supernatant 100%, and U-87 MG cell culture supernatant 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean YKL-40 concentration was determined to be 449.02 pg/mL in neat urine, 123.48 pg/mL in neat HepG2 cell culture supernatant, and 18,524 pg/mL in neat U-87 MG cell culture supernatant.
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean YKL-40 concentration was determined to be 42.74 ng/mL with a range of 16.87 – 78.32 ng/mL.
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Sandwich ELISA - Human YKL-40 ELISA Kit (ab255719)To learn more about the advantages of recombinant antibodies see here.
