Human YKL-40 Antibody Pair - BSA and Azide free (ab256754)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Human
Overview
-
Product name
Human YKL-40 Antibody Pair - BSA and Azide free
See all YKL-40/CHI3L1 kits -
Assay type
ELISA set -
Range
23.44 pg/ml - 1500 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
The Antibody Pair can be used to quantify Human YKL-40. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab255719), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
-
Notes
KD (M) kon(1/Ms) kdis(1/s) Human YKL-40 Capture Antibody 2.64 X 10-10 1.59 X 105 4.20 X 10-5 Human YKL-40 Detector Antibody 4.64 X 10-10 1.13 X 105 5.22 X 10-5 -
Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Human YKL-40 Capture Antibody (unconjugated) 1 x 100µg Human YKL-40 Detector Antibody (unconjugated) 1 x 100µg -
Research areas
-
Function
Carbohydrate-binding lectin with a preference for chitin. May play a role in defense against pathogens, or in tissue remodeling. May play an important role in the capacity of cells to respond to and cope with changes in their environment. -
Tissue specificity
Present in activated macrophages, articular chondrocytes, synovial cells as well as in liver. Undetectable in muscle tissues, lung, pancreas, mononuclear cells, or fibroblasts. -
Involvement in disease
A genetic variation in CHI3L1 is associated with susceptibility to asthma-related traits type 7 (ASRT7) [MIM:611960]. Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing and dyspnea, bronchial hyperresponsiveness (BHR) as assessed by methacholine challenge test, serum IgE levels, atopy, and atopic dermatitis. -
Sequence similarities
Belongs to the glycosyl hydrolase 18 family. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
-
Alternative names
- 39 kDa synovial protein
- ASRT7
- Cartilage glycoprotein 39
see all -
Database links
- Entrez Gene: 1116 Human
- Omim: 601525 Human
- SwissProt: P36222 Human
- Unigene: 382202 Human
Images
-
Sandwich ELISA - Human YKL-40 Antibody Pair - BSA and Azide free (ab256754)To learn more about the advantages of recombinant antibodies see here.
