Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human YKL-40 Antibody Pair - BSA and Azide free (ab256754)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Human

Overview

  • Product name

    Human YKL-40 Antibody Pair - BSA and Azide free
    See all YKL-40/CHI3L1 kits

  • Assay type

    ELISA set

  • Range

    23.44 pg/ml - 1500 pg/ml

  • Species reactivity

    Reacts with: Human

  • Product overview

    The Antibody Pair can be used to quantify Human YKL-40. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab255719), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


    Download SDS here.

  • Notes

      KD (M) kon(1/Ms) kdis(1/s)
    Human YKL-40 Capture Antibody 2.64 X 10-10 1.59 X 105 4.20 X 10-5
    Human YKL-40 Detector Antibody 4.64 X 10-10 1.13 X 105 5.22 X 10-5

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.

  • Carrier free

    Yes
  • Components 10 x 96 tests
    Human YKL-40 Capture Antibody (unconjugated) 1 x 100µg
    Human YKL-40 Detector Antibody (unconjugated) 1 x 100µg

  • Research areas

  • Function

    Carbohydrate-binding lectin with a preference for chitin. May play a role in defense against pathogens, or in tissue remodeling. May play an important role in the capacity of cells to respond to and cope with changes in their environment.

  • Tissue specificity

    Present in activated macrophages, articular chondrocytes, synovial cells as well as in liver. Undetectable in muscle tissues, lung, pancreas, mononuclear cells, or fibroblasts.

  • Involvement in disease

    A genetic variation in CHI3L1 is associated with susceptibility to asthma-related traits type 7 (ASRT7) [MIM:611960]. Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing and dyspnea, bronchial hyperresponsiveness (BHR) as assessed by methacholine challenge test, serum IgE levels, atopy, and atopic dermatitis.

  • Sequence similarities

    Belongs to the glycosyl hydrolase 18 family.

  • Post-translational
    modifications

    Glycosylated.

  • Cellular localization

    Secreted > extracellular space.
  • Information by UniProt

  • Alternative names

    • 39 kDa synovial protein
    • ASRT7
    • Cartilage glycoprotein 39
    • CGP-39
    • CGP39
    • CH3L1_HUMAN
    • CHI3L1
    • chitinase
    • chitinase 3 like 1
    • chitinase 3 like 1 (cartilage glycoprotein 39)
    • Chitinase 3 like protein 1 precursor
    • Chitinase-3-like protein 1
    • Chondrocyte protein YKL40
    • GP 39
    • GP-39
    • GP39
    • HC gp39
    • HCGP 3P
    • hCGP-39
    • HCgp39
    • YKL 40
    • YKL-40
    • YKL40
    • YYL 40
    see all

  • Database links

    Images

    • Sandwich ELISA - Human YKL-40 Antibody Pair - BSA and Azide free (ab256754)
      Sandwich ELISA - Human YKL-40 Antibody Pair - BSA and Azide free (ab256754)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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