Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Tuesday March 02, 2021

Anti-YKL-40/CHI3L1 antibody [EPR19078-157] (ab255297)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19078-157] to YKL-40/CHI3L1
Suitable for: WB, IHC-P, Flow Cyt
Reacts with: Human

Product name

Anti-YKL-40/CHI3L1 antibody [EPR19078-157]
See all YKL-40/CHI3L1 primary antibodies
Description

Rabbit monoclonal [EPR19078-157] to YKL-40/CHI3L1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human spleen tissue lysate; THP-1 (Human monocytic leukemia monocyte), U-87 MG ( Human glioblastoma-astrocytoma epithelial cell), MG-63 (Human osteosarcoma fibroblast), Caco-2 (Human colorectal adenocarcinoma epithelial cell), 293T (Human embryonic kidney epithelial cell), and Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates; IHC-P: Human spleen and tonsil tissue. Flow: THP-1 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19078-157
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] (ab255297)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling YKL-40/CHI3L1 with ab255297 at 1/250 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on neutrophils of human spleen is observed.

The section was incubated with ab255297 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20mins.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Flow Cytometry - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] (ab255297)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized THP-1 (human monocytic leukemia cell line) cells labelling YKL-40/CHI3L1 with ab255297 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Western blot - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] (ab255297)

All lanes : Anti-YKL-40/CHI3L1 antibody [EPR19078-157] (ab255297) at 1/1000 dilution

Lane 1 : Human spleen tissue lysate
Lane 2 : THP-1 (Human monocytic leukemia monocyte) whole cell lysate
Lane 3 : U-87 MG ( Human glioblastoma-astrocytoma epithelial cell) whole cell lysate
Lane 4 : MG-63 (Human osteosarcoma fibroblast) whole cell lysate
Lane 5 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 6 : 293T (Human embryonic kidney epithelial cell) whole cell lysate
Lane 7 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 43 kDa
Observed band size: 42 kDa
why is the actual band size different from the predicted?
Additional bands at: 27 kDa. We are unsure as to the identity of these extra bands.

Exposure time: 60 seconds

Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

The expression profile observed in 293T and Jurkat are consistent with the literature (PMID: 19767768,PMID: 16472595).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] (ab255297)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling YKL-40/CHI3L1 with ab255297 at 1/250 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on macrophages in human tonsil germinal center is observed. The section was incubated with ab255297 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Western blot - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] (ab255297)

All lanes : Anti-YKL-40/CHI3L1 antibody [EPR19078-157] (ab255297) at 1/1000 dilution

Lane 1 : Human spleen lysate
Lane 2 : THP-1 (human monocytic leukemia cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lane 1 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 43 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?

Blocking/Diluting buffer and concentration: 5% NFDM/TBST

Exposure time: 3 minutes

The molecular weight observed is consistent with what has been described in the literature (PMID 19414556).
Anti-YKL-40/CHI3L1 antibody [EPR19078-157] (ab255297)

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