Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-YKL-40/CHI3L1 antibody [EPR19078-157] - BSA and Azide free (ab255864)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19078-157] to YKL-40/CHI3L1 - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-YKL-40/CHI3L1 antibody [EPR19078-157] - BSA and Azide free
    See all YKL-40/CHI3L1 primary antibodies

  • Description

    Rabbit monoclonal [EPR19078-157] to YKL-40/CHI3L1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human spleen tissue lysate; THP-1 (Human monocytic leukemia monocyte), U-87 MG ( Human glioblastoma-astrocytoma epithelial cell), MG-63 (Human osteosarcoma fibroblast), Caco-2 (Human colorectal adenocarcinoma epithelial cell), 293T (Human embryonic kidney epithelial cell), and Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates; IHC-P: Human spleen and tonsil tissue. Flow: THP-1 cells.

  • General notes

    Ab255864 is the carrier-free version of ab255297. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255864 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] - BSA and Azide free (ab255864)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] - BSA and Azide free (ab255864)

    Immunohistochemical analysis of paraffin-embedded human spleen  tissue labeling YKL-40/CHI3L1 with ab255297 at 1/250 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on neutrophils of human spleen is observed.

    The section was incubated with ab255297 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255297).

  • Flow Cytometry - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] - BSA and Azide free (ab255864)
    Flow Cytometry - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] - BSA and Azide free (ab255864)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized THP-1 (human monocytic leukemia cell line) cells labelling YKL-40/CHI3L1 with ab255297 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255297).

  • Western blot - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] - BSA and Azide free (ab255864)
    Western blot - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] - BSA and Azide free (ab255864)
    All lanes : Anti-YKL-40/CHI3L1 antibody [EPR19078-157] (ab255297) at 1/1000 dilution

    Lane 1 : Human spleen tissue lysate
    Lane 2 : THP-1 (Human monocytic leukemia monocyte) whole cell lysate
    Lane 3 : U-87 MG ( Human glioblastoma-astrocytoma epithelial cell) whole cell lysate
    Lane 4 : MG-63 (Human osteosarcoma fibroblast) whole cell lysate
    Lane 5 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
    Lane 6 : 293T (Human embryonic kidney epithelial cell) whole cell lysate
    Lane 7 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 43 kDa
    Observed band size: 42 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 27 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 60 seconds


    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile observed in 293T and Jurkat are consistent with the literature (PMID: 19767768,PMID: 16472595).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255297).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] - BSA and Azide free (ab255864)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [EPR19078-157] - BSA and Azide free (ab255864)

    Immunohistochemical analysis of paraffin-embedded human tonsil  tissue labeling YKL-40/CHI3L1 with ab255297 at 1/250 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on macrophages in human tonsil germinal center is observed. The section was incubated with ab255297 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255297).

  • Anti-YKL-40/CHI3L1 antibody [EPR19078-157] - BSA and Azide free (ab255864)
    Anti-YKL-40/CHI3L1 antibody [EPR19078-157] - BSA and Azide free (ab255864)

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