Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ZAP70 (phospho Y292) antibody [EPR1073] - BSA and Azide free (ab247483)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1073] to ZAP70 (phospho Y292) - BSA and Azide free
  • Suitable for: Dot blot, ICC, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-ZAP70 (phospho Y292) antibody [EPR1073] - BSA and Azide free
    See all ZAP70 primary antibodies

  • Description

    Rabbit monoclonal [EPR1073] to ZAP70 (phospho Y292) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody only detects ZAP70 when phosphorylated at Tyrosine 292.

  • Tested applications

    Suitable for: Dot blot, ICC, Flow Cyt, WBmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247483 is the carrier-free version of ab76501 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247483 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ZAP70 (phospho Y292) antibody [EPR1073] - BSA and Azide free (ab247483)
    Western blot - Anti-ZAP70 (phospho Y292) antibody [EPR1073] - BSA and Azide free (ab247483)
    All lanes : Anti-ZAP70 (phospho Y292) antibody [EPR1073] (ab76501) at 1/5000 dilution

    Lane 1 : Untreated Jurkat (human acute T cell leukemia) whole cell lysate
    Lane 2 : Jurkat (human acute T cell leukemia) treated with pervanadate whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 70 kDa
    Observed band size: 70 kDa


    Exposure time: 3 minutes


    This data was developed using ab76501, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Dot Blot - Anti-ZAP70 (phospho Y292) antibody [EPR1073] - BSA and Azide free (ab247483)
    Dot Blot - Anti-ZAP70 (phospho Y292) antibody [EPR1073] - BSA and Azide free (ab247483)
    This data was developed using ab76501, the same antibody clone in a different buffer formulation.Dot blot analysis of varying amounts of ZAP70 (pY292) phospho peptide (Lane 1) and ZAP70 non-phospho peptide (Lane 2) using ab76501 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).Exposure time: 3 minutes.
  • Immunocytochemistry - Anti-ZAP70 (phospho Y292) antibody [EPR1073] - BSA and Azide free (ab247483)
    Immunocytochemistry - Anti-ZAP70 (phospho Y292) antibody [EPR1073] - BSA and Azide free (ab247483)
    This data was developed using ab76501, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of untreated and pervanadate treated Jurkat (human acute T cell leukemia) cells labelling ZAP70 (phospho Y292) with ab76501 at a dilution of 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Treated samples were starved overnight followed by incubation with 1mM pervanadate for 30 minutes. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. Negative control using secondary antibody only.Image shows increased cytoplasmic staining after pervanadate treatment.
  • Flow Cytometry - Anti-ZAP70 (phospho Y292) antibody [EPR1073] - BSA and Azide free (ab247483)
    Flow Cytometry - Anti-ZAP70 (phospho Y292) antibody [EPR1073] - BSA and Azide free (ab247483)
    This data was developed using ab76501, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized Jurkat cells, untreated (green), pervanadate treated (red) stained with ab76501, at 1/20 dilution (blue) and pervanadate-treated Jurkat cells using the same antibody preincubated with phospho- ZAP70 (Tyr 292) peptide (blue) or non-phospho- ZAP70 (Tyr 292)).
  • Anti-ZAP70 (phospho Y292) antibody [EPR1073] - BSA and Azide free (ab247483)
    Anti-ZAP70 (phospho Y292) antibody [EPR1073] - BSA and Azide free (ab247483)

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