Anti-ZAP70 (phospho Y319) antibody (ab264260)
Key features and details
- Rabbit polyclonal to ZAP70 (phospho Y319)
- Suitable for: IP, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ZAP70 (phospho Y319) antibody
See all ZAP70 primary antibodies -
Description
Rabbit polyclonal to ZAP70 (phospho Y319) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIP Human
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Immunogen
Synthetic peptide within Human ZAP70 (phospho Y319). The exact sequence is proprietary. NP_001070.2
Database link: P43403 -
Positive control
- IP: Jurkat (treated with hydrogen peroxide) whole cell lysate. Flow cyt: Jurkat (treated with hydrogen peroxide) cells.
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General notes
This antibody has not performed satisfactorily when used for WB of phospho ZAP70 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of ZAP70.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-ZAP70 (phospho Y319) antibody (ab264260)
1.5% PFA-fixed, 90% methonol permeabilized Jurkat (human T cell leukemia cell line from peripheral blood) (treated with hydrogen peroxide for 3 minutes) cells labeling ZAP70 (phospho Y319) with ab264260 at 1 μg / 106 cells in 150 μL (blue) compared to an isotype anti-KLH control in hydrogen peroxide-treated cells (black) and ab264260 in untreated cells (red).
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Immunoprecipitation - Anti-ZAP70 (phospho Y319) antibody (ab264260)ZAP70 (phospho Y319) was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) (treated with hydrogen peroxide)whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264260 at 3 μg/mg lysate. Western blot was performed on the immunoprecipitates using an anti-ZAP70 antibody ab245411 at 1 μg/ml.
Lane 1: ab264260 IP in Jurkat (treated) whole cell lysate.
Lane 2: Control IgG IP in Jurkat (treated) whole cell lysate.
Exposure time: 3 minutes.
