Anti-ZAP70 antibody [YE291] - BSA and Azide free (ab247258)
![Anti-ZAP70 antibody [YE291] - BSA and Azide free (ab247258)](https://www.abcam.com/ps/products/247/ab247258/Images/ab247258-456680-antiZAP70-jurkat-raji-western-blot.jpg "Anti-ZAP70 antibody [YE291] - BSA and Azide free (ab247258)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [YE291] to ZAP70 - BSA and Azide free
- Suitable for: ICC, IHC-P, WB, Flow Cyt, IP
- Reacts with: Human
Overview
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Product name
Anti-ZAP70 antibody [YE291] - BSA and Azide free
See all ZAP70 primary antibodies -
Description
Rabbit monoclonal [YE291] to ZAP70 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IHC-P, WB, Flow Cyt, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: Jurkat cells
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General notes
Ab247258 is the carrier-free version of ab32429. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab247258 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
YE291 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ZAP70 antibody [YE291] - BSA and Azide free (ab247258)All lanes : Anti-ZAP70 antibody [YE291] (ab32429) at 1/1000 dilution
All lanes :
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed)
Predicted band size: 70 kDaAnti-GAPDH antibody, ab8245 (1/20000) was used as a primary antibody for the loading control and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed, ab216776 (1/10000) was used as a loading control secondary antibody.
Lanes 1-2: Merged signal (red and green). Green – ab32429 observed at 70 kDa. Red - loading control ab8245 observed at 36 kDa.
ab32429 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.The expression profile observed in Raji is consistent with the literature (PMID: 25275600).
Negative control: Raji (PMID: 25275600)
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![Flow Cytometry - Anti-ZAP70 antibody [YE291] - BSA and Azide free (ab247258)](https://www.abcam.com/ps/products/247/ab247258/Images/ab247258-383738-anti-zap70-antibody-ye291-flow-cytometry-human-jurkat-cell.jpg)
Flow Cytometry - Anti-ZAP70 antibody [YE291] - BSA and Azide free (ab247258) -
![Anti-ZAP70 antibody [YE291] - BSA and Azide free (ab247258)](https://www.abcam.com/ps/products/247/ab247258/Images/ab247258-2-benefits-of-recombinant-antibodies.png)
Anti-ZAP70 antibody [YE291] - BSA and Azide free (ab247258)
