Anti-CD86 antibody [C86/1146] (ab220188)
![Anti-CD86 antibody [C86/1146] (ab220188)](https://www.abcam.com/ps/products/220/ab220188/Images/ab220188-5-anti-cd86-antibody-c86-1146-immunohisochemistry-melanoma-human.jpg "Anti-CD86 antibody [C86/1146] (ab220188)")
Key features and details
- Mouse monoclonal [C86/1146] to CD86
- Suitable for: ICC, IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD86 antibody [C86/1146]
See all CD86 primary antibodies -
Description
Mouse monoclonal [C86/1146] to CD86 -
Host species
Mouse -
Tested applications
Suitable for: ICC, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant full length protein corresponding to Human CD86.
Database link: P42081 -
Positive control
- WB: Daudi cell lysate; IHC-P: Human melanoma tissue; ICC: Raji cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
ab220188 is purified from Bioreactor Concentrate by Protein A/G column chromatography. -
Clonality
Monoclonal -
Clone number
C86/1146 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [C86/1146] (ab220188)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human melanoma tissue labelling CD86 with ab220188 at 2 µg/mL for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes.
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![Western blot - Anti-CD86 antibody [C86/1146] (ab220188)](https://www.abcam.com/ps/products/220/ab220188/Images/ab220188-277571-anti-cd86-antibody-c86-1146-western-blot.jpg)
Western blot - Anti-CD86 antibody [C86/1146] (ab220188)Anti-CD86 antibody [C86/1146] (ab220188) at 1 µg + Daudi cell lysate
Predicted band size: 38 kDa
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![Immunocytochemistry - Anti-CD86 antibody [C86/1146] (ab220188)](https://www.abcam.com/ps/products/220/ab220188/Images/ab220188-4-anti-cd86-antibody-c86-1146-immunocytochemistry-raji-human.jpg)
Immunocytochemistry - Anti-CD86 antibody [C86/1146] (ab220188)Immunofluorescence analysis of Raji (Human Burkitt's lymphoma cell line) cells labeling CD86 with ab220188 at 2 µg/mL followed by Goat anti-Mouse IgG-CF488 (green). The nuclear counterstain is Reddot (red).
