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Immunology Innate Immunity Macrophage / Inflamm.

Anti-PU.1/Spi1 antibody (ab230336)

Anti-PU.1/Spi1 antibody (ab230336)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to PU.1/Spi1
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-PU.1/Spi1 antibody
    See all PU.1/Spi1 primary antibodies
  • Description

    Rabbit polyclonal to PU.1/Spi1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide corresponding to Human PU.1/Spi1 aa 200 to the C-terminus.
    Database link: P17947

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: WiDr whole cell lysate. IHC-P: Human spleen tissue. ICC/IF: NIH/3T3 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Adaptive Immunity
    • B Cells
    • Non-CD
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Splicing
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Ets
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors

Images

  • Western blot - Anti-PU.1/Spi1 antibody (ab230336)
    Western blot - Anti-PU.1/Spi1 antibody (ab230336)
    Anti-PU.1/Spi1 antibody (ab230336) at 1/1000 dilution + WiDr cell lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 31 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody (ab230336)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody (ab230336)

    Paraffin-embedded, formalin fixed human spleen tissue stained for PU.1/Spi1 using ab230336 at 5μg/ml in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-PU.1/Spi1 antibody (ab230336)
    Immunocytochemistry/ Immunofluorescence - Anti-PU.1/Spi1 antibody (ab230336)

    4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryo fibroblast cell line) cells stained for PU.1/Spi1 using ab230336 at 1/25 dilution in ICC/IF.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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