Anti-MDM2 antibody - ChIP Grade (ab226939)
Key features and details
- Rabbit polyclonal to MDM2 - ChIP Grade
- Suitable for: WB, IP, IHC-P, ChIP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-MDM2 antibody - ChIP Grade
See all MDM2 primary antibodies -
Description
Rabbit polyclonal to MDM2 - ChIP Grade -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ChIP HumanIHC-P HumanIP HumanWB MouseHuman
-
Immunogen
Recombinant fragment within Human MDM2 (internal sequence). The exact sequence is proprietary.
Database link: Q00987 -
Positive control
- WB: Neuro-2a, GL261, C8D30, NIH/3T3, RAW 264.7, BCL-1, C2C12, Jurkat, Raji and NCIH-929 whole cell extracts; MDM2 transfected HEK-293T whole cell extract; HepG2, starved for 16 hours, whole cell extract. IP: Jurkat whole cell extract. IHC-P: Human lung and lung cancer tissues. ChIP: PC-3 chromatin extract.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-MDM2 antibody - ChIP Grade (ab226939)All lanes : Anti-MDM2 antibody - ChIP Grade (ab226939) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : MDM2 transfected HEK-293T whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 55 kDa7.5% SDS-PAGE gel.
-
Immunoprecipitation - Anti-MDM2 antibody - ChIP Grade (ab226939)MDM2 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract with 4 µg ab226939. Western blot was performed from the immunoprecipitate using ab226939 at 1/500 dilution.
Lane 1: Jurkat whole cell extract 30 μg (Input).
Lane 2: Control IP in Jurkat whole cell extract with 4 μg of preimmune Rabbit IgG.
Lane 3: ab226939 IP in Jurkat whole cell extract.
7.5 % SDS-PAGE gel.
-
Western blot - Anti-MDM2 antibody - ChIP Grade (ab226939)All lanes : Anti-MDM2 antibody - ChIP Grade (ab226939) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell extract
Lane 3 : NHI-H929 whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 55 kDa7.5% SDS-PAGE gel.
-
Western blot - Anti-MDM2 antibody - ChIP Grade (ab226939)All lanes : Anti-MDM2 antibody - ChIP Grade (ab226939) at 1/1000 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell extract
Lane 2 : GL261 whole cell extract
Lane 3 : C8D30 whole cell extract
Lane 4 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract
Lane 5 : BCL-1 whole cell extract
Lane 6 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract
Lane 7 : C2C12 (mouse myoblast cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 55 kDa7.5% SDS-PAGE gel.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDM2 antibody - ChIP Grade (ab226939)Paraffin-embedded human lung cancer tissue stained for MDM2 with ab226939 at 1/500 dilution in immunohistochemical analysis.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDM2 antibody - ChIP Grade (ab226939)Paraffin-embedded human lung tissue stained for MDM2 with ab226939 at 1/500 dilution in immunohistochemical analysis.
-
Western blot - Anti-MDM2 antibody - ChIP Grade (ab226939)All lanes : Anti-MDM2 antibody - ChIP Grade (ab226939) at 1/3000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line), starved for 16 hours, whole cell extract
Lane 2 : HepG2, starved for 16 hours and treated with 1 uM staurosporine for 24 hours, whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 55 kDa7.5% SDS-PAGE gel.
-
ChIP - Anti-MDM2 antibody - ChIP Grade (ab226939)Cross-linked ChIP was performed with PC-3 (human prostate adenocarcinoma cell line) chromatin extract and 5 μg of either control rabbit IgG or ab226939. The precipitated DNA was detected by PCR with primer set targeting to PSA promoter.
