Human VEGF Receptor 2 ELISA Kit (ab213476)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 10 pg/ml
- Range: 117 pg/ml - 7500 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human VEGF Receptor 2 ELISA Kit
See all VEGF Receptor 2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Human serum 8 2.5% Inter-assay Sample n Mean SD CV% Human serum 3 5.8% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
10 pg/ml -
Range
117 pg/ml - 7500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 97 95% - 98% Serum 96 94% - 97% Hep Plasma 97 95% - 99% EDTA Plasma 95 89% - 98% Cit plasma 114 109% - 122% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human VEGF Receptor 2 ELISA Kit (ab213476) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of VEGF Receptor 2 protein in cell culture supernatant, serum, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human VEGF Receptor 2 with 10 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
VEGF R2 (vascular endothelial growth factor receptor 2) is 1356 amino acids long and is a class III receptor tyrosine kinase. VEGF R2 acts as a cell surface receptor for VEGFA, VEGFC, and VEGFD, where binding of these VEGFs to VEGF R2 activates several signaling cascades, including activation of PLCG1 (leading to the activation of protein kinase C), the MAP kinase signaling pathways, and the AKT1 signaling pathway. Furthermore, VEGF R2 plays an essential role in the regulation of angiogenesis, vascular development and permeability, embryonic hematopoiesis, and reorganization of the actin cytoskeleton. Finally, VEGF R2 is a major growth factor for endothelial cells and is the main facilitator of VEGF-induced endothelial proliferation, differentiation, and migration.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human VEGF Receptor 2 Capture Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 20X Human VEGF Receptor 2 Detector Antibody 1 x 300µl Antibody Diluent 4BI 1 x 6ml Human VEGF Receptor 2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NBS 1 x 2ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. -
Involvement in disease
Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- CD309
- CD309 antigen
- EC 2.7.10.1
see all -
Database links
- Entrez Gene: 3791 Human
- Omim: 191306 Human
- SwissProt: P35968 Human
- Unigene: 479756 Human
Images
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Other - Human VEGF Receptor 2 ELISA Kit (ab213476)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human VEGF Receptor 2 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of human VEGF Receptor 2 standard curve in Sample Diluent 3.75BS.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native VEGF Receptor 2 in human serum, plasma citrate, and plasma EDTA samples.The concentrations of VEGF Receptor 2 were measured in duplicate, interpolated from the VEGF Receptor 2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 7.5%, plasma (citrate) 7.5%, and plasma (EDTA) 7.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF Receptor 2 concentration was determined to be 60 ng/mL in serum, 42 ng/mL in plasma (citrate) and 49 ng/mL in plasma (EDTA).
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Interpolated concentrations of native VEGF Receptor 2 in human plasma heparin, and spiked recombinant VEGF Receptor 2 in HUVEC culture supernatant samples.The concentrations of VEGF Receptor 2 were measured in duplicate, interpolated from the VEGF Receptor 2 standard curve and corrected for sample dilution. Undiluted samples are as follows: plasma (heparin) 12.5% and HUVEC culture supernatant 80%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF Receptor 2 concentration was determined to be 44 ng/mL in plasma (heparin). Native signal of HUVEC culture supernatant at 80% was determined to be 0.4 ng/mL.
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Serum from ten individual healthy human male donors was measured in duplicate.Serum from ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF Receptor 2 concentration was determined to be 2.37 ng/mL with a range of 1.19 – 3.23 ng/mL.
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Sandwich ELISA - Human VEGF Receptor 2 ELISA Kit (ab213476)To learn more about the advantages of recombinant antibodies see here.
