Anti-CPS1 antibody (ab3682)
Key features and details
- Rabbit polyclonal to CPS1
- Suitable for: ICC/IF, WB
- Reacts with: African green monkey
- Isotype: IgG
Overview
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Product name
Anti-CPS1 antibody
See all CPS1 primary antibodies -
Description
Rabbit polyclonal to CPS1 -
Host species
Rabbit -
Specificity
When the antibody is used at 1/2000 some lower molecular weight bands appear in COS-7 cells, the extent of which is significantly reduced when the antibody is used at 1/10,000. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: African green monkey -
Immunogen
Full length native protein (purified) (Rat).
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General notes
OMIM reference: 114010 Alternative name: carbamoylphosphate synthetase 1, CAD
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CPS1 antibody (ab3682)
Lane 1: 5 ul of 2ml lysate cos-7 cells transfected with full length CPS 1. Before IP
Lane 2: cos-7 cells transfected with full length CPS 1. IP with 5 ul of pre-immune serum
Lane 3: cos-7 cells transfected with full length CPS 1. IP with 2 ul of polyclonal antibody anti CPS 1 (ab3682)
Lane 4: cos-7 cells transfected with full length CPS 1. IP with 5 ul of polyclonal antibody anti CPS 1 (ab3682)
Lane 5: 5 ul of 2ml lysate cos-7 cells transfected with empty vector (pCMV5-tr). Before IP
Lane 6: cos-7 cells transfected with empty vector (pCMV5-tr). IP with 5 ul of polyclonal antibody anti CPS 1 (ab3682)
Lane 7: 5 ul of 2ml lysate untransfected cos-7 cells. Before IP
Lane 8: untransfected cos-7 cells. IP with 5 ul of polyclonal antibody anti CPS 1 (ab3682) Ab3682 can IP CPS1 transiently expressed in COS-7 cells.
Lane 1: 5 ul of 2ml lysate cos-7 cells transfected with full length CPS 1. Before IP
Lane 2: cos-7 cells transfected with full length CPS 1. IP -
Immunocytochemistry/ Immunofluorescence - Anti-CPS1 antibody (ab3682)CPS-1 localisation is consistent with the reticulated pattern of liver mitonchondria.
Rabbit polyclonal to CPS-1 (ab3682), at 1/5000 on COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV5-CPS 1 plasmid (Corvi, M.M and Berthiaume, L.G. unpublished data).
Secondary: donkey anti-rabbit (Texas Red).
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Western blot - Anti-CPS1 antibody (ab3682)Western blot using Rabbit polyclonal to CPS 1 (ab3682). All lanes loaded with 100 ng of purified CPS 1
Lane 1: ab3682 1/500
Lane 2: ab3682 1/1000
Lane 3: ab3682 1/2000 Western blot using Rabbit polyclonal to CPS 1 (ab3682).
All lanes loaded with 100 ng of purified CPS 1
Lane 1: ab3682 1/500
Lane 2: ab3682 1/1000
Lane 3: ab3682 1/2000
