Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CPS1 antibody [EPR7493-3] (ab129076)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7493-3] to CPS1
  • Suitable for: WB, IHC-P, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CPS1 antibody [EPR7493-3]
    See all CPS1 primary antibodies

  • Description

    Rabbit monoclonal [EPR7493-3] to CPS1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human CPS1 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • WB: Human fetal liver, mouse liver and rat liver tissue lysates and HeLa cell lysate. IHC-P: Human liver tissue. Flow Cyt: HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CPS1 antibody [EPR7493-3] (ab129076)
    Western blot - Anti-CPS1 antibody [EPR7493-3] (ab129076)
    All lanes : Anti-CPS1 antibody [EPR7493-3] (ab129076) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : CPS1 knockout HeLa cell lysate
    Lane 3 : Human liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 165 kDa
    Observed band size: 165 kDa



    Lanes 1-3: Merged signal (red and green). Green - ab129076 observed at 165 kDa. Red - loading control, ab8245 observed at 37 kDa.

    ab129076 Anti-CPS1 antibody [EPR7493-3] was shown to specifically react with CPS1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261809 (knockout cell lysate ab257121) was used. Wild-type and CPS1 knockout samples were subjected to SDS-PAGE. ab129076 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-CPS1 antibody [EPR7493-3] (ab129076)
    Western blot - Anti-CPS1 antibody [EPR7493-3] (ab129076)
    All lanes : Anti-CPS1 antibody [EPR7493-3] (ab129076) at 1/1000 dilution

    Lane 1 : Fetal liver cell lysate
    Lane 2 : Mouse liver cell lysate
    Lane 3 : Rat liver cell lysate
    Lane 4 : HeLa cell lysate

    Predicted band size: 165 kDa
    Observed band size: 150 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPS1 antibody [EPR7493-3] (ab129076)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPS1 antibody [EPR7493-3] (ab129076)

    ab129076, at 1/100 dilution staining CPS1 in formalin fixed paraffin embedded Human liver tissue by immunohistochemistry

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-CPS1 antibody [EPR7493-3] (ab129076)
    Flow Cytometry - Anti-CPS1 antibody [EPR7493-3] (ab129076)

    Flow cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling CPS1 (red) with ab129076 at a 1/20 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Anti-CPS1 antibody [EPR7493-3] (ab129076)
    Anti-CPS1 antibody [EPR7493-3] (ab129076)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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