Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Tuesday March 02, 2021

Anti-CPS1 antibody [EPR7493-3] (ab129076)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR7493-3] to CPS1
Suitable for: WB, IHC-P, Flow Cyt
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-CPS1 antibody [EPR7493-3]
See all CPS1 primary antibodies
Description

Rabbit monoclonal [EPR7493-3] to CPS1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide within Human CPS1 aa 100-200. The exact sequence is proprietary.
Positive control
- WB: Human fetal liver, mouse liver and rat liver tissue lysates and HeLa cell lysate. IHC-P: Human liver tissue. Flow Cyt: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR7493-3
Isotype

IgG
Research areas

Western blot - Anti-CPS1 antibody [EPR7493-3] (ab129076)

All lanes : Anti-CPS1 antibody [EPR7493-3] (ab129076) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : CPS1 knockout HeLa cell lysate
Lane 3 : Human liver tissue lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 165 kDa
Observed band size: 165 kDa

Lanes 1-3: Merged signal (red and green). Green - ab129076 observed at 165 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab129076 Anti-CPS1 antibody [EPR7493-3] was shown to specifically react with CPS1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261809 (knockout cell lysate ab257121) was used. Wild-type and CPS1 knockout samples were subjected to SDS-PAGE. ab129076 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-CPS1 antibody [EPR7493-3] (ab129076)

All lanes : Anti-CPS1 antibody [EPR7493-3] (ab129076) at 1/1000 dilution

Lane 1 : Fetal liver cell lysate
Lane 2 : Mouse liver cell lysate
Lane 3 : Rat liver cell lysate
Lane 4 : HeLa cell lysate

Predicted band size: 165 kDa
Observed band size: 150 kDa
why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPS1 antibody [EPR7493-3] (ab129076)

ab129076, at 1/100 dilution staining CPS1 in formalin fixed paraffin embedded Human liver tissue by immunohistochemistry

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cytometry - Anti-CPS1 antibody [EPR7493-3] (ab129076)

Flow cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling CPS1 (red) with ab129076 at a 1/20 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).
Anti-CPS1 antibody [EPR7493-3] (ab129076)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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