Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NME2 antibody [EPR8351] (ab131329)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8351] to NME2
  • Suitable for: ICC, WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human, Chinese hamster

Overview

  • Product name

    Anti-NME2 antibody [EPR8351]
    See all NME2 primary antibodies

  • Description

    Rabbit monoclonal [EPR8351] to NME2

  • Host species

    Rabbit

  • Specificity

    The immunogen used to raise this antibody shares 85% homology with human NME1. Cross-reactivity with this protein has not been confirmed experimentally.

    The mouse, rat and Chinese hamster recommendation is based on the WB results. We do not guarantee IHC-P for mouse, rat and Chinese hamster.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Chinese hamster
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human NME2 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • WB: LnCaP, PC-12, CHO, 293T, Human spleen and Human thyroid lysates. Rat and mouse kidney tissue lysates; IHC: Human kidney tissue and human cerebellum tissue; Flow Cyt: HEK293 cells; ICC: CHO-S cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NME2 antibody [EPR8351] (ab131329)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NME2 antibody [EPR8351] (ab131329)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling NME2 with purified ab131329 at 1/500 dilution (0.22 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-NME2 antibody [EPR8351] (ab131329)
    Western blot - Anti-NME2 antibody [EPR8351] (ab131329)
    All lanes : Anti-NME2 antibody [EPR8351] (ab131329) at 1/1000 dilution (purified)

    Lane 1 : Mouse kidney lysate
    Lane 2 : Rat kidney lysate
    Lane 3 : CHO-S (Chinese hamster ovary epithelial cell) whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 17 kDa
    Observed band size: 17 kDa

  • Immunocytochemistry - Anti-NME2 antibody [EPR8351] (ab131329)
    Immunocytochemistry - Anti-NME2 antibody [EPR8351] (ab131329)

    Immunocytochemistry/ Immunofluorescence analysis of CHO-S (Chinese hamster ovary epithelial cell) cells labeling NME2 with purified ab131329 at 1/50 dilution (2.18 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry - Anti-NME2 antibody [EPR8351] (ab131329)
    Flow Cytometry - Anti-NME2 antibody [EPR8351] (ab131329)

    Flow Cytometry analysis of HEK-293 (Human embryonickidney epithelial cell) cells labeling NME2 with purified ab131329 at 1/20 dilution (5 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Western blot - Anti-NME2 antibody [EPR8351] (ab131329)
    Western blot - Anti-NME2 antibody [EPR8351] (ab131329)
    All lanes : Anti-NME2 antibody [EPR8351] (ab131329) at 1/1000 dilution (purified)

    Lane 1 : 293T (Human embryonic kidney epithelial cell) whole cell lysate
    Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 17 kDa
    Observed band size: 17 kDa

  • Western blot - Anti-NME2 antibody [EPR8351] (ab131329)
    Western blot - Anti-NME2 antibody [EPR8351] (ab131329)
    All lanes : Anti-NME2 antibody [EPR8351] (ab131329) at 1/1000 dilution (unpurified)

    Lane 1 : LnCaP cell lysate
    Lane 2 : PC-12 cell lysate
    Lane 3 : CHO cell lysate
    Lane 4 : 293T cell lysate
    Lane 5 : Human spleen lysate
    Lane 6 : Human thyroid lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 17 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NME2 antibody [EPR8351] (ab131329)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NME2 antibody [EPR8351] (ab131329)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling NME2 with unpurified ab131329 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-NME2 antibody [EPR8351] (ab131329)
    OI-RD Scanning - Anti-NME2 antibody [EPR8351] (ab131329)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-NME2 antibody [EPR8351] (ab131329)
    Anti-NME2 antibody [EPR8351] (ab131329)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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