Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Septin 2 antibody [EPR12122] (ab187654)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12122] to Septin 2
  • Suitable for: ICC/IF, WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Septin 2 antibody [EPR12122]
    See all Septin 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR12122] to Septin 2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Jurkat, MCF7, U-87 MG, mouse NIH 3T3, mouse RAW 264.7, rat PC12 cell lysates; Human fetal brain lysate; Human breast carcinoma tissue; MCF7, Jurkat and mouse U-87 MG cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR12122

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Septin 2 antibody [EPR12122] (ab187654)
    Immunocytochemistry/ Immunofluorescence - Anti-Septin 2 antibody [EPR12122] (ab187654)

    Immunofluorescence analysis of MCF7 cells labeling Septin 2 using ab187654 at 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/200 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde. Counterstain: DAPI.

  • Western blot - Anti-Septin 2 antibody [EPR12122] (ab187654)
    Western blot - Anti-Septin 2 antibody [EPR12122] (ab187654)
    All lanes : Anti-Septin 2 antibody [EPR12122] (ab187654) at 1/10000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : U-87 MG cell lysate
    Lane 4 : Human fetal brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 41 kDa
    Observed band size: 41 kDa

  • Western blot - Anti-Septin 2 antibody [EPR12122] (ab187654)
    Western blot - Anti-Septin 2 antibody [EPR12122] (ab187654)
    All lanes : Anti-Septin 2 antibody [EPR12122] (ab187654) at 1/1000 dilution

    Lane 1 : mouse Raw 264.7 cell lysate
    Lane 2 : rat PC12 cell lysate
    Lane 3 : mouse NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 41 kDa
    Observed band size: 41 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 2 antibody [EPR12122] (ab187654)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 2 antibody [EPR12122] (ab187654)

    Immunohistochemical analysis of paraffin-embedded, Human breast carcinoma tissue labeling Septin 2 with ab187654 at a 1/1000 dilution. Counter stained with hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Septin 2 antibody [EPR12122] (ab187654)
    Immunocytochemistry/ Immunofluorescence - Anti-Septin 2 antibody [EPR12122] (ab187654)

    Immunofluorescence analysis of, paraformaldehyde-fixed, MCF-7 cell line labeling Septin 2 with ab187654 at a 1/100 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) was used at a 1/200 (left panel). In blue DAPI staining (right panel).

  • Flow Cytometry - Anti-Septin 2 antibody [EPR12122] (ab187654)
    Flow Cytometry - Anti-Septin 2 antibody [EPR12122] (ab187654)

    Flow cytometry analysis of paraformaldehyde-fixed Jurkat cells labeling Septin 2 with ab187654 at a 1/120 dilution and secondary antibody FITC goat anti-rabbit IgG (red) at a 1/150 dilution, or negative control rabbit IgG (green).

  • Anti-Septin 2 antibody [EPR12122] (ab187654)
    Anti-Septin 2 antibody [EPR12122] (ab187654)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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