Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Septin 2 antibody [EPR12122] (ab187654)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12122] to Septin 2
Suitable for: ICC/IF, WB, IHC-P, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-Septin 2 antibody [EPR12122]
See all Septin 2 primary antibodies
Description

Rabbit monoclonal [EPR12122] to Septin 2
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Jurkat, MCF7, U-87 MG, mouse NIH 3T3, mouse RAW 264.7, rat PC12 cell lysates; Human fetal brain lysate; Human breast carcinoma tissue; MCF7, Jurkat and mouse U-87 MG cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR12122
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-Septin 2 antibody [EPR12122] (ab187654)

Immunofluorescence analysis of MCF7 cells labeling Septin 2 using ab187654 at 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/200 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde. Counterstain: DAPI.
Western blot - Anti-Septin 2 antibody [EPR12122] (ab187654)

All lanes : Anti-Septin 2 antibody [EPR12122] (ab187654) at 1/10000 dilution

Lane 1 : Jurkat cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : U-87 MG cell lysate
Lane 4 : Human fetal brain lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 41 kDa
Observed band size: 41 kDa
Western blot - Anti-Septin 2 antibody [EPR12122] (ab187654)

All lanes : Anti-Septin 2 antibody [EPR12122] (ab187654) at 1/1000 dilution

Lane 1 : mouse Raw 264.7 cell lysate
Lane 2 : rat PC12 cell lysate
Lane 3 : mouse NIH 3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 41 kDa
Observed band size: 41 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 2 antibody [EPR12122] (ab187654)

Immunohistochemical analysis of paraffin-embedded, Human breast carcinoma tissue labeling Septin 2 with ab187654 at a 1/1000 dilution. Counter stained with hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Septin 2 antibody [EPR12122] (ab187654)

Immunofluorescence analysis of, paraformaldehyde-fixed, MCF-7 cell line labeling Septin 2 with ab187654 at a 1/100 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) was used at a 1/200 (left panel). In blue DAPI staining (right panel).
Flow Cytometry - Anti-Septin 2 antibody [EPR12122] (ab187654)

Flow cytometry analysis of paraformaldehyde-fixed Jurkat cells labeling Septin 2 with ab187654 at a 1/120 dilution and secondary antibody FITC goat anti-rabbit IgG (red) at a 1/150 dilution, or negative control rabbit IgG (green).
Anti-Septin 2 antibody [EPR12122] (ab187654)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com