Anti-Septin 2 antibody - C-terminal (ab185998)
Key features and details
- Rabbit polyclonal to Septin 2 - C-terminal
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Septin 2 antibody - C-terminal
See all Septin 2 primary antibodies -
Description
Rabbit polyclonal to Septin 2 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
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Immunogen
Synthetic peptide within Human Septin 2 aa 300 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_001008491.1.
Database link: Q15019 -
Positive control
- HeLa, 293T, Jurkat, mouse TCMK1 and mouse NIH 3T3 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab185998 was affinity purified using an epitope specific to Septin 2 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Septin 2 antibody - C-terminal (ab185998)All lanes : Anti-Septin 2 antibody - C-terminal (ab185998) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 41 kDa
Exposure time: 30 seconds
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Immunoprecipitation - Anti-Septin 2 antibody - C-terminal (ab185998)Detection of Human Septin 2 by Western Blot of Immunoprecipitates of 293T
whole cell lysates (1 mg for IP; 20% of IP loaded).
Lane 1: ab185998 used for IP at 6 μg/mg lysate.
Lane 2: Control IgG.
For blotting, ab185998 was used at 1 μg/ml.
Detection: Chemiluminescence with an exposure time of 3 seconds.
