Anti-RPE65 antibody (ab67042)
Key features and details
- Rabbit polyclonal to RPE65
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-RPE65 antibody
See all RPE65 primary antibodies -
Description
Rabbit polyclonal to RPE65 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Chicken, Cow, Human, Xenopus laevis, Non human primates, Zebrafish
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab99880) -
Positive control
- This antibody gave a positive signal in the Mouse Retina Tissue Lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab67042 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 61 kDa). Target
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Function
Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association. -
Tissue specificity
Retinal pigment epithelium specific. -
Involvement in disease
Defects in RPE65 are the cause of Leber congenital amaurosis type 2 (LCA2) [MIM:204100]. LCA designates a clinically and genetically heterogeneous group of childhood retinal degenerations, generally inherited in an autosomal recessive manner. Affected infants have little or no retinal photoreceptor function as tested by electroretinography. LCA represents the most common genetic cause of congenital visual impairment in infants and children.
Defects in RPE65 are the cause of retinitis pigmentosa type 20 (RP20) [MIM:613794]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP20 inheritance is autosomal dominant. -
Sequence similarities
Belongs to the carotenoid oxygenase family. -
Post-translational
modificationsPalmitoylation by LRAT regulates ligand binding specificity; the palmitoylated form (membrane form) specifically binds all-trans-retinyl-palmitate, while the soluble unpalmitoylated form binds all-trans-retinol (vitamin A). -
Cellular localization
Cytoplasm. Cell membrane. Attached to the membrane by a lipid anchor when palmitoylated (membrane form), soluble when unpalmitoylated. - Information by UniProt
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Database links
- Entrez Gene: 395700 Chicken
- Entrez Gene: 282043 Cow
- Entrez Gene: 6121 Human
- Entrez Gene: 19892 Mouse
- Entrez Gene: 89826 Rat
- Entrez Gene: 379961 Xenopus laevis
- Omim: 180069 Human
- SwissProt: Q9YGX2 Chicken
see all -
Alternative names
- All-trans-retinyl-palmitate hydrolase antibody
- LCA 2 antibody
- LCA2 antibody
see all
Images
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Western blot - Anti-RPE65 antibody (ab67042)Anti-RPE65 antibody (ab67042) at 1 µg/ml + Mouse Retina Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutes -
Western blot - Anti-RPE65 antibody (ab67042)This image is courtesy of an anonymous AbreviewAll lanes : Anti-RPE65 antibody (ab67042) at 1/1000 dilution
Lane 1 : Lepomis macrochirus (blue gill)RPE Lysate at 10 µl
Lane 2 : Lepomis macrochirus (blue gill)RPE Lysate at 5 µl
Secondary
All lanes : HRP congugated Goat anti-rabbit at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 61 kDa
Fish species: Lepomis macrochirus (blue gill)
Datasheets and documents
References (2)
ab67042 has been referenced in 2 publications.
- Hazim RA et al. Differentiation of RPE cells from integration-free iPS cells and their cell biological characterization. Stem Cell Res Ther 8:217 (2017). ICC ; Human . PubMed: 28969679
- Liu C et al. Subretinal injection of amyloid-ß peptide accelerates RPE cell senescence and retinal degeneration. Int J Mol Med 35:169-76 (2015). PubMed: 25385658
Images
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Western blot - Anti-RPE65 antibody (ab67042)Anti-RPE65 antibody (ab67042) at 1 µg/ml + Mouse Retina Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutes
-
Western blot - Anti-RPE65 antibody (ab67042) This image is courtesy of an anonymous AbreviewAll lanes : Anti-RPE65 antibody (ab67042) at 1/1000 dilution
Lane 1 : Lepomis macrochirus (blue gill)RPE Lysate at 10 µl
Lane 2 : Lepomis macrochirus (blue gill)RPE Lysate at 5 µl
Secondary
All lanes : HRP congugated Goat anti-rabbit at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 61 kDa
Fish species: Lepomis macrochirus (blue gill)
