Anti-RPE65 antibody [401.8B11.3D9] (ab13826)
![Anti-RPE65 antibody [401.8B11.3D9] (ab13826)](https://www.abcam.com/ps/products/13/ab13826/Images/ab13826-21087-anti-rpe65-antibody-4018b113d9-western-blot.jpg "Anti-RPE65 antibody [401.8B11.3D9] (ab13826)")
Key features and details
- Mouse monoclonal [401.8B11.3D9] to RPE65
- Suitable for: WB, ICC, IHC-Fr
- Reacts with: Mouse, Cow, Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-RPE65 antibody [401.8B11.3D9]
See all RPE65 primary antibodies -
Description
Mouse monoclonal [401.8B11.3D9] to RPE65 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanIHC-Fr MouseWB CowAfrican green monkey
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Immunogen
Tissue, cells or virus corresponding to RPE65. Bovine RPE microsomal membrane proteins.
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Positive control
- WB: Recombinant human RPE protein expressed in COS-7 cells and bovine RPE membrane fraction. IHC-Fr: Mouse retina tissue. ICC: ARPE19 cells.
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General notes
Abcam is committed to meeting high quality standards of ethical manufacturing and has decided to discontinue this product on 31st March 2021 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.
Expression in primary culture of RPE cells is undetectable in Western blot after day 14. Established cell lines are similarly devoid of RPE65 protein, as indicated in western blot. However, mRNA levels are detectable by Northern for at least 7 weeks. (see reference Hamel, C.P., et al. Molecular Cloning and expression of RPE65 a novel retinal pigment epithelium-specific microsomal protein that is post-transcriptionally regulated in vitro. JBC. 268(21): 15751-15757, 1993.)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Ascites -
Purification notes
Purified from ascites. -
Clonality
Monoclonal -
Clone number
401.8B11.3D9 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-RPE65 antibody [401.8B11.3D9] (ab13826)All lanes : Anti-RPE65 antibody [401.8B11.3D9] (ab13826) at 1/5000 dilution
Lane 1 : Recombinant human RPE-transfected COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate at 20 µg
Lane 2 : Bovine retinal pigment epithelium membrane fraction at 5 µg
Secondary
All lanes : Alkaline phosphatase conjugated goat-anti Mouse IgG
Observed band size: 60 kDa why is the actual band size different from the predicted?NBT/BCIP substrate
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![Immunocytochemistry - Anti-RPE65 antibody [401.8B11.3D9] (ab13826)](https://www.abcam.com/ps/products/13/ab13826/Images/ab13826-308489-anti-rpe65-antibody-4018b113d9-immunofluorescence.jpg)
Immunocytochemistry - Anti-RPE65 antibody [401.8B11.3D9] (ab13826)ARPE19 (spontaneously arising human retinal pigment epithelia cell line) cells stained for RPE65 (green) using ab13826 (1/100 dilution) in ICC/IF.
Cells were fixed in 4% PFA for 10 minutes, permeabilized in PBS containing 0.2% Triton X-100 (PBS-T) for 10 minutes, then blocked for 1 hour in 10% normal goat serum containing 1% BSA in PBS-T. Incubated with primary antibody at 4°C.
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![Immunohistochemistry (Frozen sections) - Anti-RPE65 antibody [401.8B11.3D9] (ab13826)](https://www.abcam.com/ps/products/13/ab13826/Images/ab13826-21086-anti-rpe65-antibody-4018b113d9-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-RPE65 antibody [401.8B11.3D9] (ab13826)Frozen sectioned mouse retina tissue stained for RPE65 with ab13826 at a 1/250 dilution in immunohistochemical analysis.
