Flavin Adenine Dinucleotide (FAD) Assay Kit (ab204710)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Sample type: Cell culture media, Cell Lysate, Plasma, Serum, Tissue Lysate
- Sensitivity: 1 nmol/well
Overview
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Product name
Flavin Adenine Dinucleotide (FAD) Assay Kit -
Detection method
Colorimetric/Fluorometric -
Sample type
Serum, Plasma, Cell Lysate, Cell culture media, Tissue Lysate -
Assay type
Enzyme activity -
Sensitivity
Species reactivity
Reacts with: Mammals, Other speciesProduct overview
Flavin Adenine Dinucleotide (FAD) Assay Kit (ab204710) is an assay where FAD functions as the cofactor of an oxidase which catalyze the formation of a product that reacts with OxiRed probe generating color and fluorescence. FAD can be detected by either colorimetric (OD=570 nm) or fluorometric (Ex/Em=535/587 nm) methods. The kit provides a rapid, simple, ultra-sensitive, and reliable test suitable for high throughput assay of FAD. The lower limit of detection is less than 1 nM FAD.
FAD assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with microplate reader until absorbance reaches 1.8 at 570 nm (or stop before then)Notes
Flavin Adenine Dinucleotide (FAD) is a redox cofactor which plays an important role in metabolism. FAD exists in different redox states and cycles between FAD, FADH and FADH2. The primary sources of reduced FAD in eukaryotic metabolism are the citric acid cycle and the beta oxidation reaction pathways.
Platform
Microplate readerProperties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests FAD Assay Buffer 1 x 25ml FAD Enzyme Mix (lyophilized) 1 vial FAD Standard (1 nmol, lyophilized) 1 vial OxiRed Probe (in DMSO) 1 x 200µl Stop Solution 1 x 1.2ml
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests FAD Assay Buffer 1 x 25ml FAD Enzyme Mix (lyophilized) 1 vial FAD Standard (1 nmol, lyophilized) 1 vial OxiRed Probe (in DMSO) 1 x 200µl Stop Solution 1 x 1.2ml
Images
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Flavin Adenine Dinucleotide (FAD) Assay Kit (ab204710)
Typical FAD Standard Curve (Fluorometric Assay).
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Flavin Adenine Dinucleotide (FAD) Assay Kit (ab204710)Typical FAD Standard Curve (Colorimetric Assay).