Anti-Lamin A + Lamin C antibody (ab227176)
Key features and details
- Rabbit polyclonal to Lamin A + Lamin C
- Suitable for: WB, ICC/IF, IP, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Lamin A + Lamin C antibody
See all Lamin A + Lamin C primary antibodies -
Description
Rabbit polyclonal to Lamin A + Lamin C -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Dog, Pig, Chimpanzee, Rhesus monkey
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Immunogen
Recombinant fragment within Human Lamin A + Lamin C (internal sequence). The exact sequence is proprietary.
Database link: P02545 -
Positive control
- WB: HeLa and A431 whole cell extracts; NIH/3T3 whole cell lysate. IHC-P: Human breast carcinoma and Mahlavu xenograft tissues; mouse C2C12 xenograft tissue; rat RT2 xenograft tissue. ICC/IF: HeLa cells. IP: HeLa whole cell extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Lamin A + Lamin C antibody (ab227176)All lanes : Anti-Lamin A + Lamin C antibody (ab227176) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) (WT) cell extract
Lane 2 : Lamin A + C knockout in HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 74 kDa7.5% SDS-PAGE gel.
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Western blot - Anti-Lamin A + Lamin C antibody (ab227176)Anti-Lamin A + Lamin C antibody (ab227176) at 1/2000 dilution + A431 (human epidermoid carcinoma cell line) whole cell extract at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 74 kDa7.5% SDS-PAGE gel.
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Western blot - Anti-Lamin A + Lamin C antibody (ab227176)Anti-Lamin A + Lamin C antibody (ab227176) at 1/1000 dilution + NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 74 kDa7.5% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-Lamin A + Lamin C antibody (ab227176)Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Lamin A + C (green) using ab227176 at 1/500 dilution in ICC/IF.
Counterstain: Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (red) at 1/500 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody (ab227176)Paraffin-embedded human breast carcinoma tissue stained for Lamin A + C using ab227176 at 1/250 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody (ab227176)Paraffin-embedded human Mahlarvu xenograft tissue stained for Lamin A + C using ab227176 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody (ab227176)Paraffin-embedded mouse C2C12 xenograft tissue stained for Lamin A + C using ab227176 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody (ab227176)Paraffin-embedded rat RT2 xenograft tissue stained for Lamin A + C using ab227176 at 1/500 dilution in immunohistochemical analysis.
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Immunoprecipitation - Anti-Lamin A + Lamin C antibody (ab227176)Lamin A + C was immunoprecipitated from HeLa whole cell extract with 4 µg of ab227176. Western blot was performed from the immunoprecipitate using ab227176 at 1/500 dilution. Anti-rabbit IgG was used as secondary antibody.
Lane 1: HeLa whole cell extract 50 µg (Input).
Lane 2: Preimmune Rabbit IgG instead of ab227176 in HeLa whole cell extract.
Lane 3: ab227176 IP in HeLa whole cell extract.
