Anti-Lamin A + Lamin C antibody [EP4520-16] (ab133256)
![Anti-Lamin A + Lamin C antibody [EP4520-16] (ab133256)](https://www.abcam.com/ps/products/133/ab133256/Images/ab133256-382903-anti-lamin-a--lamin-c-antibody-ep4520-16-western-blot-hap1-wt-hap1-lmna-knockout-hela-hepg2.jpg "Anti-Lamin A + Lamin C antibody [EP4520-16] (ab133256)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP4520-16] to Lamin A + Lamin C
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Lamin A + Lamin C antibody [EP4520-16]
See all Lamin A + Lamin C primary antibodies -
Description
Rabbit monoclonal [EP4520-16] to Lamin A + Lamin C -
Host species
Rabbit -
Specificity
The antibody recognizes full length Lamin A/C and the cleaved large unit. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human Lamin A + Lamin C aa 200-300. The exact sequence is proprietary.
Database link: P02545 -
Positive control
- WB: Untreated HeLa, HeLa treated with staurosporine, HeLa treated with camptothecine, HAP1, HepG2, and Jurkat (ab7899) whole cell lysate. IHC-P: Human breast and human transitional cell carcinoma of bladder tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP4520-16 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Lamin A + Lamin C antibody [EP4520-16] (ab133256)All lanes : Anti-Lamin A + Lamin C antibody [EP4520-16] (ab133256) at 1/5000 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : LMNA knockout HAP1 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 74 kDa
Observed band size: 70-75 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab133256 observed at 70-75 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab133256 was shown to react with LMNA in wild-type HAP1 cells in Western blot. Loss of signal was observed when LMNA knockout sample was used. HAP1 wild-type and LMNA knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab133256 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [EP4520-16] (ab133256)](https://www.abcam.com/ps/products/133/ab133256/Images/ab133256-146676-anti-lamin-a-c-antibody-ep4520-16-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [EP4520-16] (ab133256)Immunohistochemical analysis of paraffin-embedded Human breast tissue labelling Lamin A with ab133256 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [EP4520-16] (ab133256)](https://www.abcam.com/ps/products/133/ab133256/Images/ab133256-146675-anti-lamin-a-c-antibody-ep4520-16-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [EP4520-16] (ab133256)Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labelling Lamin A with ab133256 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-Lamin A + Lamin C antibody [EP4520-16] (ab133256)](https://www.abcam.com/ps/products/133/ab133256/Images/ab133256-146677-anti-lamin-a-c-antibody-ep4520-16-western-blot.jpg)
Western blot - Anti-Lamin A + Lamin C antibody [EP4520-16] (ab133256)All lanes : Anti-Lamin A + Lamin C antibody [EP4520-16] (ab133256) at 1/10000 dilution
Lane 1 : HeLa treated with staurosporine cell lysate
Lanes 2 & 5 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa treated with camptothecine cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 74 kDa
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![Anti-Lamin A + Lamin C antibody [EP4520-16] (ab133256)](https://www.abcam.com/ps/products/133/ab133256/Images/ab133256-6-benefits-of-recombinant-antibodies.png)
Anti-Lamin A + Lamin C antibody [EP4520-16] (ab133256)
