Anti-EpCAM antibody [Ber-EP4] (ab7504)
![Anti-EpCAM antibody [Ber-EP4] (ab7504)](https://www.abcam.com/ps/products/7/ab7504/Images/ab7504-12961-anti-epcam-antibody-ber-ep4-immunohistochemistry.jpg "Anti-EpCAM antibody [Ber-EP4] (ab7504)")
Key features and details
- Mouse monoclonal [Ber-EP4] to EpCAM
- Suitable for: IHC-FoFr, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-EpCAM antibody [Ber-EP4]
See all EpCAM primary antibodies -
Description
Mouse monoclonal [Ber-EP4] to EpCAM -
Host species
Mouse -
Specificity
Epithelial specific antigen is cell surface glycoprotein and is broadly distributed in epithelial cells and displays a highly conserved expression in carcinomas. Epithelial specific antigen has been know to play an important role as tumor-cell marker in lymph nodes from patients with esophageal carcinoma. Epithelial specific antigen can be used to distinguish between basal cell and basosquamous carcinomas and squamous cell carcinoma of the skin. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
BALB/C mice were immunized with human breast carcinoma cell line, MCF-7.
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Positive control
- Kidney tissue sections.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: Tissue culture supernatant, 1% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
Ber-EP4 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [Ber-EP4] (ab7504)ab7504 staining EpCAM in formalin-fixed, paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry. Staining was detected using DAB.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [Ber-EP4] (ab7504)](https://www.abcam.com/ps/products/7/ab7504/Images/ab7504-12962-anti-epcam-antibody-ber-ep4-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [Ber-EP4] (ab7504) This image is courtesy of an Abreview submitted by Eva Schmelzerab7504 staining EpCAM in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% serum for 1 hour at 20°C; antigen retrieval was by heat mediation (microwave) in a commercial retrieval buffer. Samples were incubated with primary antibody (1/250 in 1% serum) for 12 hours at 4°C. A Biotin-conjugated Horse anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.
