Human FAS ELISA Kit (ab183360)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 26 pg/ml
- Range: 46.88 pg/ml - 3000 pg/ml
- Sample type: Cell culture media, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human FAS ELISA Kit
See all Fas kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Media 8 3.8% Inter-assay Sample n Mean SD CV% Media 3 9.5% -
Sample type
Cell culture supernatant, Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
26 pg/ml -
Range
46.88 pg/ml - 3000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 97 91% - 108% Serum 97 88% - 107% Cell culture media 100 94% - 105% Hep Plasma 105 98% - 111% EDTA Plasma 92 81% - 102% Cit plasma 98 90% - 105% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
As of October 15, 2019, Human FAS ELISA kit has been re-developed. We have identified new recombinant monoclonal antibodies to provide improved performance and consistency.
Human FAS ELISA kit (ab183360) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FAS protein in human cell culture supernatant, serum, and plasma samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate human FAS with 2 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits. -
Notes
FAS is a plasma membrane receptor for FASLG ligand (TNFSF6/FASLG). Upon ligand binding, the FASLG Receptor is activated and the adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) activation mediating apoptosis. FASLG-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 of the FASLG Receptor block apoptosis as they sequester the Fas ligand.
This kit uses a recombinant protein fragment corresponding to the extracellular portion (amino acid 17-172) of the full length Human Fas Receptor protein as standard sample. -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human FAS Capture Antibody 1 x 600µl 10X Human FAS Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human FAS Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). -
Tissue specificity
Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6. -
Involvement in disease
Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly. -
Sequence similarities
Contains 1 death domain.
Contains 3 TNFR-Cys repeats. -
Domain
Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- ALPS 1A
- ALPS1A
- APO 1
see all -
Database links
- Entrez Gene: 355 Human
- Omim: 134637 Human
- SwissProt: P25445 Human
- Unigene: 244139 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human FAS standard curve comparisonStandard Curve comparison between the original Human FAS SimpleStep ELISA kit and current Human FAS SimpleStep ELISA kit.
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Example of human FAS standard curve in Sample Diluent NS.The FAS standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native FAS in human serum and plasma samples.The concentrations of FAS were measured in duplicates, interpolated from the FAS standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate, EDTA, heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FAS concentration was determined to be 1,393 pg/mL in neat serum, 1,231 pg/mL in neat plasma (citrate) and 1,226 pg/mL in neat plasma (EDTA) and 1,237pg/mL in neat plasma (heparin).
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Interpolated concentrations of native FAS in human PBMC cell culture supernatant derived from cells seeded at 107 cells/mL and cultured in the presence or absence of 1.5% PHA for 36 hours.The concentrations of FAS were measured in duplicate and interpolated from the FAS standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FAS concentration was determined to be 157 pg/mL in neat stimulated PBMC cell culture supernatant and undetectable in unstimulated PBMC cell culture supernatant.
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Serum from ten individual healthy human male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FAS concentration was determined to be 1,513 pg/mL with a range of 1,050 – 2,009 pg/mL.
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Sandwich ELISA - Human FAS ELISA Kit (ab183360)To learn more about the advantages of recombinant antibodies see here.
