Mouse C Reactive Protein Matched Antibody Pair Kit (ab222280)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 125 pg/ml - 8000 pg/ml
Overview
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Product name
Mouse C Reactive Protein Matched Antibody Pair Kit
See all C Reactive Protein kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
35.7 pg/ml -
Range
125 pg/ml - 8000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse C Reactive Protein Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse C Reactive Protein.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab222511. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse C Reactive Protein Capture Antibody 2 x 50µg 1 x 50µg Mouse C Reactive Protein Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse C Reactive Protein Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. -
Tissue specificity
Found in plasma. -
Sequence similarities
Belongs to the pentaxin family.
Contains 1 pentaxin domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Pentraxin 1, short
- C reactive protein
- C reactive protein pentraxin related
see all -
Database links
- Entrez Gene: 12944 Mouse
- SwissProt: P14847 Mouse
- Unigene: 28767 Mouse
Images
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Mouse C Reactive Protein standard curve
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse C Reactive Protein Matched Antibody Pair Kit (ab222280)To learn more about the advantages of recombinant antibodies see here.
