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Mouse C Reactive Protein ELISA Kit (ab222511)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 17 pg/ml
  • Range: 156.25 pg/ml - 10000 pg/ml
  • Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse C Reactive Protein ELISA Kit
    See all C Reactive Protein kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 5 2.4%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 2.1%

  • Sample type

    Serum, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    17 pg/ml

  • Range

    156.25 pg/ml - 10000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 111 109% - 113%
    Tissue Extracts 115 103% - 122%
    Hep Plasma 113 110% - 117%
    EDTA Plasma 107 103% - 109%
    Cit plasma 108 90% - 117%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse
    Does not react with: Cow

  • Product overview

    Mouse C Reactive Protein ELISA Kit (ab222511) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of C Reactive Protein protein in cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse C Reactive Protein with 31 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    C Reactive Protein displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. C Reactive Protein can interact with DNA and histones and it may scavenge nuclear material released from damaged circulating cells. C Reactive Protein is secreted; it forms a homopentamer pentaxin (or pentraxin) which have a discoid arrangement of 5 non-covalently bound subunits. C Reactive Protein binds 2 calcium ions per subunit. The concentration of C Reactive Protein in plasma increases greatly during acute phase response to tissue injury, infection or other inflammatory stimuli. It is induced by IL1/interleukin-1and IL6//interleukin-6.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Other - Mouse C Reactive Protein ELISA Kit (ab222511)
    Other - Mouse C Reactive Protein ELISA Kit (ab222511)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of mouse C Reactivte Protein standard curve in Sample Diluent NS + Enhancer.
    Example of mouse C Reactivte Protein standard curve in Sample Diluent NS + Enhancer.

    The C Reactive Protein standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Example of mouse C Reactive Protein standard curve in 1X Cell Extraction Buffer PTR.
    Example of mouse C Reactive Protein standard curve in 1X Cell Extraction Buffer PTR.

    The C Reactive Protein standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native C Reactive Protein in mouse serum and plasma samples.
    Interpolated concentrations of native C Reactive Protein in mouse serum and plasma samples.

    The concentrations of C Reactive Protein were measured in duplicates, interpolated from the C Reactive Protein standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:400, plasma (citrate) 1:800, plasma (heparin) 1:400, and plasma (EDTA) 1:1600. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean C Reactive Protein concentration was determined to be 2854 ng/mL in neat serum, 5469 ng/mL in neat plasma (citrate), 3358 ng/mL in neat plasma (heparin), and 7251 ng/mL in neat plasma (EDTA).

  • Interpolated concentrations of native C Reactive Protein in mouse liver tissue extract.
    Interpolated concentrations of native C Reactive Protein in mouse liver tissue extract.

    Based on a 200 μg/mL extract load and mouse heart tissue extract based on a 500 μg/mL extract load. The concentrations of C Reactive Protein were measured in duplicate and interpolated from the C Reactive Protein standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean C Reactive Protein concentration was determined to be 7416 pg/mL in mouse liver tissue extract and 5978 pg/mL in mouse heart tissue extract.

  • Sandwich ELISA - Mouse C Reactive Protein ELISA Kit (ab222511)
    Sandwich ELISA - Mouse C Reactive Protein ELISA Kit (ab222511)
    To learn more about the advantages of recombinant antibodies see here.

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