Monkey CCL4 ELISA Kit (ab242229)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.8 pg/ml
- Range: 9.4 pg/ml - 600 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Monkey, Cynomolgus monkey, Rhesus monkey
Overview
-
Product name
Monkey CCL4 ELISA Kit
See all CCL4/MIP-1 beta kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% cell culture 8 9.7% Inter-assay Sample n Mean SD CV% cell culture 3 10.3% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.8 pg/ml -
Range
9.4 pg/ml - 600 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 106 102% - 110% Serum 111 103% - 124% Cell culture media 116 102% - 131% Hep Plasma 83 72% - 90% EDTA Plasma 93 81% - 107% Cit plasma 87 76% - 96% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Monkey, Cynomolgus monkey, Rhesus monkey -
Product overview
Monkey CCL4 ELISA Kit (ab242229) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CCL4 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Monkey CCL4 with 1.8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
Notes
CCL4 (MIP-1 beta) is a monokine with inflammatory and chemokinetic properties. CCL4 binds to CCR5 and is one of the major HIV suppressive factors produced by CD8+ T cells. Recombinant CCL4 induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form CCL4 retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T cells. This form of CCL4 is also a ligand for CCR1 and CCR2 isoform B. Structurally, CCL4 forms a heterodimer with MIP-1 alpha and is secreted from peripheral blood lymphocytes.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml 20X Monkey CCL4 Capture Antibody 1 x 600µl 20X Monkey CCL4 Detector Antibody 1 x 600µl 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Monkey CCL4 Lyophilized recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
-
Function
Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsN-terminal processed form MIP-1-beta(3-69) is produced by proteolytic cleavage after secretion from peripheral blood lymphocytes. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- MIP 1 beta
- Secreted protein G 26
- ACT 2
see all
Images
-
Other - Monkey CCL4 ELISA Kit (ab242229)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Example of comparison of human and non-human primate CCL4.Standard Curve comparison between human and non-human primate CCL4. -
Example of monkey CCL4 standard curve in 1X Cell Extraction Buffer PTR.The CCL4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of native CCL4 in rhesus macaque serum and plasma samples.The concentrations of CCL4 were measured in duplicates, interpolated from the CCL4 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum neat, plasma (citrate) neat, plasma (heparin) neat, and plasma (EDTA) neat. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL4 concentration was determined to be 140.2 pg/mL in neat serum, 101.6 pg/mL in neat plasma (citrate), 55.1 in neat plasma (heparin), and 109.5 pg/mL in neat plasma (EDTA). -
Interpolated concentrations of native CCL4 in rhesus macaque stimulated peripheral blood mononuclear cell (PBMC) culture supernatant samples.Rhesus macaque PBMCs were cultured in the presence or absence of 50 ng/mL PMA and 1 µg/mL Ionomycin for 24 hours. The concentrations of CCL4 were measured in duplicates, interpolated from the CCL4 standard curves and corrected for sample dilution. Undiluted samples are as follows: stimulate PBMC supernatant 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL4 concentration was determined to be 35.89 ng/mL in neat stimulated rhesus macaque PBMC cell culture supernatant and undetectable in unstimulated PBMC cell culture supernatant and naive cell culture media (not shown).
-
Comparison of rhesus macaque and cynomolgus monkey serum, plasma, and PMA + Ionomycin stimulated PBMC cell culture supernatants (24 hours).The concentrations of CCL4 were measured in duplicates, interpolated from the CCL4 standard curves and corrected for sample dilution. The interpolated dilution factor corrected values in neat samples are plotted (mean +/- SD, n=2). -
Sandwich ELISA - Monkey CCL4 ELISA Kit (ab242229)To learn more about the advantages of recombinant antibodies see here.
