Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Immunology Innate Immunity Macrophage / Inflamm.

Anti-Pyrin (phospho S205) antibody [EPR19567] - BSA and Azide free (ab222490)

Anti-Pyrin (phospho S205) antibody [EPR19567] - BSA and Azide free (ab222490)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19567] to Pyrin (phospho S205) - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse

You may also be interested in

Product image
Anti-Flagellin antibody (ab93713)
Product image
Anti-IL-17RB antibody (ab229320)
Product image
Recombinant Human NLRP3 (mutated K232A) protein (Tagged) (ab271629)
Product image
Human MSP ELISA Kit (MST1) (ab278126)

Overview

  • Product name

    Anti-Pyrin (phospho S205) antibody [EPR19567] - BSA and Azide free
    See all Pyrin primary antibodies
  • Description

    Rabbit monoclonal [EPR19567] to Pyrin (phospho S205) - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data
  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: DC2.4 whole cell lysate expressing Pyrin (MEFV).
  • General notes

    Ab222490 is the carrier-free version of ab201784. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab222490 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19567
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Binding Proteins

Images

  • Western blot - Anti-Pyrin (phospho S205) antibody [EPR19567] - BSA and Azide free (ab222490)
    Western blot - Anti-Pyrin (phospho S205) antibody [EPR19567] - BSA and Azide free (ab222490)
    All lanes : Anti-Pyrin (phospho S205) antibody [EPR19567] (ab201784) at 1/1000 dilution

    Lane 1 : Cell lysate of DC2.4 (Mouse immature dendritic cell line) expressing mPyrin (MEFV)
    Lane 2 : Cell lysate of DC2.4 (Mouse immature dendritic cell line) expressing mPyrin (MEFV) and mPyrin (MEFV) is dephosphorylated by stimulation with TcdA

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 86 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 second


    This data was developed using ab201784, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The lysates were kindly provided by Feng Shao, National institute of biological sciences Beijing, China.

  • Anti-Pyrin (phospho S205) antibody [EPR19567] - BSA and Azide free (ab222490)
    Anti-Pyrin (phospho S205) antibody [EPR19567] - BSA and Azide free (ab222490)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Pyrin (phospho S205) antibody [EPR19567] - BSA and Azide free (ab222490)

  •  
  • Product image

    Anti-Pyrin (phospho S241) antibody [EPR19570] (ab200420)

    Applications: Dot, WB

  •  
  • Product image

    Anti-Pyrin antibody [EPR18676] - BSA and Azide free (ab222484)

    Applications: IP, WB

  •  
  • Product image

    Anti-Pyrin antibody [EPR18676] (ab195975)

    Applications: IP, WB

  •  
  • Product image

    Anti-Pyrin antibody [EPR18675] (ab214772)

    Applications: IP, WB

  •  
  • Product image

    Anti-Pyrin (phospho S241) antibody [EPR19570] - BSA and Azide free (ab222488)

    Applications: Dot, WB

  •  
  • Product image

    Anti-Pyrin (phospho S205) antibody [EPR19567] (ab201784)

    Applications: WB

  •  
  • Product image

    Anti-Pyrin antibody [EPR18675] - BSA and Azide free (ab223132)

    Applications: IP, WB

Clear all

Recently viewed products

  •  
  • Product image

    Recombinant Human GTSF1 protein (ab180332)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.