Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Pyrin antibody [EPR18675] (ab214772)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18675] to Pyrin
Suitable for: WB, IP
Knockout validated
Reacts with: Mouse

Product name

Anti-Pyrin antibody [EPR18675]
See all Pyrin primary antibodies
Description

Rabbit monoclonal [EPR18675] to Pyrin
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Mouse

WB
Mouse

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: DC2.4 stable Pyrin expression whole cell lysate; Bone marrow derived-macrophage of wild type C57/B6 mice, untreated or stimulated with LPS. IP: DC2.4 stable Pyrin expression whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Mouse
WB	Mouse

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18675
Isotype

IgG
Research areas

Western blot - Anti-Pyrin antibody [EPR18675] (ab214772)

All lanes : Anti-Pyrin antibody [EPR18675] (ab214772) at 1/1000 dilution

Lane 1 : DC2.4 (Mouse immature dendritic cell line) whole cell lysate
Lane 2 : DC2.4 (Mouse immature dendritic cell line) stable mPyrin expression whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 86 kDa
Observed band size: 110 kDa
why is the actual band size different from the predicted?

Exposure time: 1 second

Blocking/Dilution buffer: 5% NFDM/TBST.

The cells were kindly provided by our collaborator, Feng Shao, National institute of biological sciences, Beijing.
Western blot - Anti-Pyrin antibody [EPR18675] (ab214772)

All lanes : Anti-Pyrin antibody [EPR18675] (ab214772) at 1/1000 dilution

Lane 1 : Bone marrow derived-macrophage of Pyrin -/- mice
Lane 2 : Bone marrow derived-macrophage of Pyrin -/- mice stimulated with LPS
Lane 3 : Bone marrow derived-macrophage of wild type C57/B6 mice
Lane 4 : Bone marrow derived-macrophage of wild type C57/B6 mice stimulated with LPS

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 86 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?

Exposure time: 1 minute

Blocking/Dilution buffer: 5% NFDM/TBST.

The lysate is kindly provided by our collaborator, Feng Shao, National institute of biological sciences, Beijing.
Immunoprecipitation - Anti-Pyrin antibody [EPR18675] (ab214772)

Pyrin was immunoprecipitated from 0.35 mg of DC2.4 (Mouse immature dendritic cell line) stable mPyrin expression whole cell lysate with ab214772 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab214772 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

Lane 1: ab214772 whole cell lysate from DC2.4 stable mPyrin expression cells 10µg (Input).

Lane 2: ab214772 IP in whole cell lysate from DC2.4 stable mPyrin expression cells.

Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab214772 in whole cell lysate from DC2.4 stable mPyrin expression cells.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 30 seconds.
Anti-Pyrin antibody [EPR18675] (ab214772)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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