Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Pyrin antibody [EPR18676] (ab195975)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18676] to Pyrin
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Pyrin antibody [EPR18676]
    See all Pyrin primary antibodies

  • Description

    Rabbit monoclonal [EPR18676] to Pyrin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Bone marrow derived-macrophage of wild type C57/B6 mice, untreated and stimulated with LPS or TNF alpha; DC2.4 stable expression whole cell lysate. IP: DC2.4 stable expression whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Pyrin antibody [EPR18676] (ab195975)
    Western blot - Anti-Pyrin antibody [EPR18676] (ab195975)
    All lanes : Anti-Pyrin antibody [EPR18676] (ab195975) at 1/1000 dilution

    Lane 1 : Bone marrow derived-macrophage of Pyrin -/- mice
    Lane 2 : Bone marrow derived-macrophage of Pyrin -/- mice stimulated with LPS
    Lane 3 : Bone marrow derived-macrophage of Pyrin -/- mice stimulated with TNF alpha
    Lane 4 : Bone marrow derived-macrophage of wild type C57/B6 mice
    Lane 5 : Bone marrow derived-macrophage of wild type C57/B6 mice stimulated with LPS
    Lane 6 : Bone marrow derived-macrophage of wild type C57/B6 mice stimulated with TNF alpha

    Lysates/proteins at 5 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L) at 1/5000 dilution

    Predicted band size: 86 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?


    Exposure time: 60 seconds


    Blocking/Dilution buffer: 5% milk/TBST.

    The data is kindly provided by our collaborator, Feng Shao, National institute of biological sciences, Beijing.

  • Western blot - Anti-Pyrin antibody [EPR18676] (ab195975)
    Western blot - Anti-Pyrin antibody [EPR18676] (ab195975)
    All lanes : Anti-Pyrin antibody [EPR18676] (ab195975) at 1/50 dilution

    Lane 1 : Bone marrow derived-macrophage Pyrin -/- mice
    Lane 2 : Bone marrow derived-macrophage Pyrin -/- mice stimulated with LPS
    Lane 3 : Bone marrow derived-macrophage of wild type C57/B6 mice
    Lane 4 : Bone marrow derived-macrophage of wild type C57/B6 mice stimulated with LPS

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/2000 dilution

    Predicted band size: 86 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The lysate is kindly provided by our collaborator, Feng Shao, National institute of biological sciences, Beijing.

  • Western blot - Anti-Pyrin antibody [EPR18676] (ab195975)
    Western blot - Anti-Pyrin antibody [EPR18676] (ab195975)
    All lanes : Anti-Pyrin antibody [EPR18676] (ab195975) at 1/50 dilution

    Lane 1 : DC2.4 (Mouse immature dendritic cell line) whole cell lysate
    Lane 2 : DC2.4 (Mouse immature dendritic cell line) stable mPyrin expression whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/2000 dilution

    Predicted band size: 86 kDa
    Observed band size: 110 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The cells were kindly provided by our collaborator, Feng Shao, National institute of biological sciences, Beijing.

  • Immunoprecipitation - Anti-Pyrin antibody [EPR18676] (ab195975)
    Immunoprecipitation - Anti-Pyrin antibody [EPR18676] (ab195975)

    Pyrin was immunoprecipitated from DC2.4 (Human immature dendritic cell line) stable Pyrin expression whole cell lysate with ab195975 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab195975 at 1/1000 dilution. Goat anti-Rabbit IgG (H+L), was used as secondary antibody at 1/5000 dilution.

    Lane 1: DC2.4 whole cell lysate.

    Lane 2: DC2.4 stable Pyrin expression whole cell lysate.

    Lane 3: DC2.4 stable Pyrin expression whole cell lysate.

    Lane 4: ab195975 IP in DC2.4 whole cell lysate.

    Lane 5: ab195975 IP in DC2.4 stable Pyrin expression whole cell lysate.

    Lane 6: Mock IP (without ab195975) in DC2.4 stable Pyrin expression whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • Anti-Pyrin antibody [EPR18676] (ab195975)
    Anti-Pyrin antibody [EPR18676] (ab195975)

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