Human Alpha 1 Acid Glycoprotein / AGP ELISA Kit (AGP) (ab108852)
Key features and details
- Sensitivity: 2.3 ng/ml
- Range: 12.5 ng/ml - 100 ng/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Alpha 1 Acid Glycoprotein / AGP ELISA Kit (AGP)
See all Alpha 1 Acid Glycoprotein/AGP kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 4.8% Inter-assay Sample n Mean SD CV% Overall 9.8% -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid -
Assay type
Sandwich (quantitative) -
Sensitivity
= 2.3 ng/ml -
Range
12.5 ng/ml - 100 ng/ml -
Recovery
= 96 %
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Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Alpha 1 Acid Glycoprotein / AGP Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of AGP levels in cell culture supernatant, saliva, milk, and urine.
An Alpha 1 Acid Glycoprotein / AGP specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Alpha 1 Acid Glycoprotein / AGP specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Alpha 1 Acid Glycoprotein / AGP captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Alpha 1 Acid Glycoprotein / AGP Antibody 1 x 120µl Alpha 1 Acid Glycoprotein / AGP Microplate (12 x 8 well strips) 1 unit Alpha 1 Acid Glycoprotein / AGP Standard 1 vial Chromogen Substrate 1 x 8ml Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
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Function
Appears to function in modulating the activity of the immune system during the acute-phase reaction. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Sequence similarities
Belongs to the calycin superfamily. Lipocalin family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- A1AG1_HUMAN
- AGP
- AGP 1
see all -
Database links
- Entrez Gene: 5004 Human
- Omim: 138600 Human
- SwissProt: P02763 Human
- Unigene: 522356 Human
Images
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Typical Standard Curve
Representative Standard Curve using ab108852.
