Human Alpha 1 acid Glycoprotein / AGP ELISA Kit (AGP) (ab108854)
Key features and details
- Sensitivity: 0.1 µg/ml
- Range: 0.063 µg/ml - 4 µg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Overview
-
Product name
Human Alpha 1 acid Glycoprotein / AGP ELISA Kit (AGP)
See all Alpha 1 Acid Glycoprotein/AGP kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5.3% Inter-assay Sample n Mean SD CV% Overall 10% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Competitive -
Sensitivity
= 0.1 µg/ml -
Range
0.063 µg/ml - 4 µg/ml -
Recovery
= 98 %
-
Assay time
3h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Alpha 1 acid Glycoprotein / AGP Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitive measurement of Alpha 1 acid Glycoprotein / AGP in plasma and serum.
An Alpha 1 acid Glycoprotein / AGP specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Alpha 1 acid Glycoprotein / AGP is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Alpha 1 acid Glycoprotein / AGP captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
-
Platform
Microplate
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 1X Biotinylated Human Alpha 1 acid Glycoprotein / AGP (Lyophilized) 1 vial 20X Wash Buffer Concentrate 1 x 30ml Alpha 1 acid Glycoprotein / AGP Microplate (12 x 8 well strips) 1 unit Alpha 1 acid Glycoprotein / AGP Standard 1 vial Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml -
Research areas
-
Function
Appears to function in modulating the activity of the immune system during the acute-phase reaction. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Sequence similarities
Belongs to the calycin superfamily. Lipocalin family. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- A1AG1_HUMAN
- AGP
- AGP 1
see all -
Database links
- Entrez Gene: 5004 Human
- Omim: 138600 Human
- SwissProt: P02763 Human
- Unigene: 522356 Human
Images
-
Competitive ELISA - Alpha1 acid Glycoprotein / AGP (ab108854)
Alpha1 acid Glycoprotein / AGP measured in various biological fluids showing quantity (ng) per mL of sample tested
-
Competitive ELISA- Alpha1 acid Glycoprotein / AGP (ab108854)Standard curve : mean of duplicates (+/-SD)
