Astana Biomed Group, an authorized Abcam distributor in Central Asia

Horse CRP ELISA Kit (ab190527)

Key features and details

  • Sensitivity: 1.198 ng/ml
  • Range: 6.25 ng/ml - 200 ng/ml
  • Sample type: Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Horse

Overview

  • Product name

    Horse CRP ELISA Kit
    See all C Reactive Protein kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall
    Inter-assay
    Sample n Mean SD CV%
    Overall

  • Sample type

    Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    1.198 ng/ml

  • Range

    6.25 ng/ml - 200 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum > 85 % - %

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Horse

  • Product overview

    Abcam’s CRP Horse ELISA kit (ab190527) is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of CRP in horse serum and plasma.

    In this assay the CRP present in samples reacts with the anti-CRP antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-CRP antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound CRP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of CRP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of CRP in the test sample. The quantity of CRP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

  • Notes

    Acute phase proteins are plasma proteins which increase in concentration following infection, inflammation or trauma. The first acute phase protein to be recognized was discovered in humans by Tillet and Frances in 1930. This C-reactive protein (CRP) is so named because it is able to effect precipitation of somatic C-polysaccharide of Streptococcus pneumonia. CRP is an alpha globulin with a mass of 110,000 to 140,000 daltons, and composed of five identical subunits, which are non-covalently assembled as a cyclic pentamer. It is synthesized in the liver and, in humans, is normally present as a trace constituent of serum at levels less that 0.3 mG/dL. The levels in serum rise quickly following acute tissue damage and also falls very rapidly once the stimulus is removed. It has been proposed that the function of CRP is to aid in complement activation, influence phagocytic cell function, and augment cell mediated cytotoxicity. Investigations over the past few years have shown that quantification of these in plasma or serum can provide valuable diagnostic information in the detection, prognosis, and monitoring of disease not only in humans, but in companion animals and farm herds as well.

  • Platform

    Microplate

Properties

Images

  • Standard Curve
    Standard Curve

    Representative standard curve using ab190527 CRP Horse ELISA Kit.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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