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Immunology Innate Immunity Macrophage / Inflamm.

Anti-C Reactive Protein antibody [EPR20063] (ab207756)

Price and availability

278 083 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-C Reactive Protein antibody [EPR20063] (ab207756)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20063] to C Reactive Protein
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-C Reactive Protein antibody [EPR20063]
    See all C Reactive Protein primary antibodies
  • Description

    Rabbit monoclonal [EPR20063] to C Reactive Protein
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human, mouse and rat serum; Rat liver and kidney lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20063
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Cardiovascular
    • Blood
    • Acute Phase Reactants
    • Immunology
    • Innate Immunity
    • Complement
    • Classical Pathway
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Cardiovascular
    • Heart
    • Apoptosis
    • Metabolism
    • Types of disease
    • Metabolic disorders

Images

  • Western blot - Anti-C Reactive Protein antibody [EPR20063] (ab207756)
    Western blot - Anti-C Reactive Protein antibody [EPR20063] (ab207756)
    Lanes 1-2 & 4 : Anti-C Reactive Protein antibody [EPR20063] (ab207756) at 1/1000 dilution
    Lane 3 : Anti-C Reactive Protein antibody [EPR20063] (ab207756) at 1/5000 dilution

    Lane 1 : Human serum
    Lane 2 : Rat liver lysate
    Lane 3 : Rat serum
    Lane 4 : Rat kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1-3 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
    Lane 4 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/10000 dilution

    Predicted band size: 25 kDa
    Observed band size: 25,30 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1/2/4: 30 seconds; Lane 3: 1 second.

    Observed MWt: 25 kDa (Human), 30 kDa (Rat-glycosylated).

    The molecular weight observed is consistent with what has been described in the literature (PMID: 7093286).

  • Western blot - Anti-C Reactive Protein antibody [EPR20063] (ab207756)
    Western blot - Anti-C Reactive Protein antibody [EPR20063] (ab207756)
    Anti-C Reactive Protein antibody [EPR20063] (ab207756) at 1/1000 dilution + Mouse serum at 10 µg

    Secondary
    VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

    Predicted band size: 25 kDa
    Observed band size: 25 kDa


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-C Reactive Protein antibody [EPR20063] (ab207756)
    Anti-C Reactive Protein antibody [EPR20063] (ab207756)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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