Anti-C Reactive Protein antibody [EPR20063] (ab207756)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20063] to C Reactive Protein
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-C Reactive Protein antibody [EPR20063]
See all C Reactive Protein primary antibodies -
Description
Rabbit monoclonal [EPR20063] to C Reactive Protein -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human, mouse and rat serum; Rat liver and kidney lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20063 -
Isotype
IgG -
Research areas
Images
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Lanes 1-2 & 4 : Anti-C Reactive Protein antibody [EPR20063] (ab207756) at 1/1000 dilution
Lane 3 : Anti-C Reactive Protein antibody [EPR20063] (ab207756) at 1/5000 dilution
Lane 1 : Human serum
Lane 2 : Rat liver lysate
Lane 3 : Rat serum
Lane 4 : Rat kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-3 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
Lane 4 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/10000 dilution
Predicted band size: 25 kDa
Observed band size: 25,30 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1/2/4: 30 seconds; Lane 3: 1 second.
Observed MWt: 25 kDa (Human), 30 kDa (Rat-glycosylated).
The molecular weight observed is consistent with what has been described in the literature (PMID: 7093286).
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Anti-C Reactive Protein antibody [EPR20063] (ab207756) at 1/1000 dilution + Mouse serum at 10 µg
Secondary
VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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