Mouse Myeloperoxidase Antibody Pair - BSA and Azide free (MPO) (ab253440)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Mouse
Overview
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Product name
Mouse Myeloperoxidase Antibody Pair - BSA and Azide free (MPO)
See all Myeloperoxidase kits -
Assay type
ELISA set -
Range
273.438 pg/ml - 17500 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
The Antibody Pair can be used to quantify Mouse Myeloperoxidase. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab275109), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Mouse Myeloperoxidase (MPO) Capture Antibody (unconjugated) 1 x 100µg Mouse Myeloperoxidase (MPO) Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. -
Involvement in disease
Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis. -
Sequence similarities
Belongs to the peroxidase family. XPO subfamily. -
Cellular localization
Lysosome. - Information by UniProt
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Alternative names
- 84 kDa myeloperoxidase
- 89 kDa myeloperoxidase
- EC 1.11.1.7
see all -
Database links
- Entrez Gene: 17523 Mouse
- SwissProt: P11247 Mouse
- Unigene: 4668 Mouse
Images
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Sandwich ELISA - Mouse Myeloperoxidase Antibody Pair - BSA and Azide free (MPO) (ab253440)To learn more about the advantages of recombinant antibodies see here.
