Mouse Myeloperoxidase ELISA Kit (MPO) (ab275109)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 117 pg/ml
- Range: 273.44 pg/ml - 17500 pg/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Myeloperoxidase ELISA Kit (MPO)
See all Myeloperoxidase kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3% Inter-assay Sample n Mean SD CV% Serum 3 6% -
Sample type
Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
117 pg/ml -
Range
273.44 pg/ml - 17500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 97 74% - 110% Cell culture media 99 96% - 100% Hep Plasma 115 108% - 120% EDTA Plasma 108 102% - 118% Cit plasma 119 110% - 126% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Myeloperoxidase ELISA kit (ab275109) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse Myeloperoxidase protein in serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Myeloperoxidase with 117 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Myeloperoxidase (MPO) is part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. MPO is a hemoprotein expressed in the neutrophils (polymorphonuclear leukocytes) and is secreted during activation. Myeloperoxidase catalyzes the oxidation of chloride ions to hypochlorus acid, which is a potent antimicrobial agent. Myeloperoxidase pro- oxidative and pro-inflammatory properties cause an increase in MPO in multiple diseases including heart disease, myocardial infraction, and multiple sclerosis. Myeloperoxidase is a clinical marker of myocardial infraction and cardiovascular disease.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Myeloperoxidase (MPO) Capture Antibody 1 x 600µl 10X Mouse Myeloperoxidase (MPO) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPR 1 x 6ml Mouse Myeloperoxidase (MPO) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 2 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. -
Involvement in disease
Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis. -
Sequence similarities
Belongs to the peroxidase family. XPO subfamily. -
Cellular localization
Lysosome. - Information by UniProt
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Alternative names
- 84 kDa myeloperoxidase
- 89 kDa myeloperoxidase
- EC 1.11.1.7
see all -
Database links
- Entrez Gene: 17523 Mouse
- SwissProt: P11247 Mouse
- Unigene: 4668 Mouse
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse Myeloperoxidase standard curve in Sample Diluent NS.The Myeloperoxidase standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Myeloperoxidase in mouse serum and plasma samples.The concentrations of Myeloperoxidase were measured in duplicates, interpolated from the Myeloperoxidase standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 7.5%, plasma (citrate) 3.8%, plasma (EDTA) 7.5% and plasma (heparin) 7.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Myeloperoxidase concentration was determined to be 138 ng/mL in neat serum, 244 ng/mL in plasma (citrate) and 124 ng/mL in neat plasma (EDTA) and 123 ng/mL in neat plasma (heparin).
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Sandwich ELISA - Mouse Myeloperoxidase ELISA Kit (MPO) (ab275109)To learn more about the advantages of recombinant antibodies see here.
