Human MIP1a ELISA Kit (ab214569)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.4 pg/ml
- Range: 1.56 pg/ml - 100 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human MIP1a ELISA Kit
See all Macrophage Inflammatory Protein 1 alpha / CCL3 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5 1.9% Inter-assay Sample n Mean SD CV% Overall 3 7.2% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.4 pg/ml -
Range
1.56 pg/ml - 100 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 101 100% - 102% Serum 99 96% - 101% Hep Plasma 97 94% - 100% EDTA Plasma 100 92% - 105% Cit plasma 95 91% - 101% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human MIP1a ELISA Kit (ab214569) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MIP1a protein in cell culture supernatant, serum, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MIP1a with 0.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Macrophage Inflammatory Protein 1-alpha (MIP1a, also known as CCL3) is a monokine with both inflammatory and chemokine properties. MIP-1-alpha can bind to CCR1, CCR4 and CCR5. In addition, it is one of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml 10X Human MIP1a Capture Antibody 1 x 600µl 10X Human MIP1a Detector Antibody 1 x 600µl Human MIP1a Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Sample Diluent NS (ab193972) 1 x 50ml Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsN-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T-cells. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C C motif chemokine 3
- CCL 3
- CCL3
see all -
Database links
- Entrez Gene: 6348 Human
- Omim: 182283 Human
- SwissProt: P10147 Human
- Unigene: 514107 Human
Images
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Other - Human MIP1a ELISA Kit (ab214569)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human MIP1a standard curve comparison data.Standard curve comparison between human MIP1a SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 24-fold increase in sensitivity.
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Example of MIP1a standard curve.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spiked MIP1a in human serum and plasma samples.The concentrations of MIP1a were measured in duplicates, interpolated from the MIP1a standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 50%, plasma (heparin) 50%) and plasma (citrate) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIP1a concentration was determined to be 5.5 pg/mL with a range of 1.3 – 12.1 pg/mL.
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Interpolated concentrations of native MIP1a in human peripheral blood monocyte (PBMC) cell culture supernatant samples.The concentrations of MIP1a were measured in duplicates, interpolated from the MIP1a standard curves and corrected for sample dilution. Undiluted samples are as follows: unstimulated 6.35% and stimulated 0.2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIP1a concentration was determined to be 0.241 ng/mL in unstimulated PBMC cell culture supernatant and 40.8 ng/mL in stimulated.
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Sandwich ELISA - Human MIP1a ELISA Kit (ab214569)To learn more about the advantages of recombinant antibodies see here.
