Anti-MAD2 antibody (ab70383)
Key features and details
- Rabbit polyclonal to MAD2
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MAD2 antibody
See all MAD2 primary antibodies -
Description
Rabbit polyclonal to MAD2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Guinea pig, Cow, Pig, Chimpanzee, Ferret, Baboon, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide mapping to a region between residues 100 and 150 of human MAD2 (according to SwissProt entry Q13257)
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Positive control
- IP: HEK293T whole cell lysate
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-MAD2 antibody (ab70383)
Detection of human MAD2 by immunoprecipitation of whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Lane 1: Rabbit polyclonal to MAD2 (ab70383) at 6 µg/mg lysate, Lane 2: Control IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. ab70386 was used at 0.1 ug/mL for Western blot.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAD2 antibody (ab70383)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling MAD2 with ab70383 at 1/1000 (1µg/ml). Detection: DAB. -
Immunocytochemistry/ Immunofluorescence - Anti-MAD2 antibody (ab70383)ICC/IF image of ab70383 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70383, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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