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Anti-MAD2 antibody (ab70385)

Anti-MAD2 antibody (ab70385)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to MAD2
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-MAD2 antibody
    See all MAD2 primary antibodies
  • Description

    Rabbit polyclonal to MAD2
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide mapping to a region between residue 150 and the C-terminus (residue 205) of Human MAD2 (SwissProt Q13257)

  • Positive control

    • NETN whole cell lysate from HeLa cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Spindle
    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Other cell division antibodies
    • Cancer
    • Cell cycle
    • Cell division

Images

  • Western blot - Anti-MAD2 antibody (ab70385)
    Western blot - Anti-MAD2 antibody (ab70385)
    Anti-MAD2 antibody (ab70385) at 1/5000 dilution + NETN whole cell lysate from HeLa cells at 50 µg

    Developed using the ECL technique.

    Predicted band size: 24 kDa
    Observed band size: 24 kDa
    Additional bands at: 58 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 1 minute
  • Immunoprecipitation - Anti-MAD2 antibody (ab70385)
    Immunoprecipitation - Anti-MAD2 antibody (ab70385)
    Detection of Human MAD2 by Immunoprecipitation in NETN whole cell lysate from HeLa cells (600 µg/IP) using ab70385 at 0.7 µg/mg for IP and at 0.2 µg/ml for subsequent WB detection. Note: bands between 50 and 60 kDa represent the IgG chains.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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