Human MIP1a Matched Antibody Pair Kit (ab216069)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 0.14 pg/ml - 100 pg/ml
Overview
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Product name
Human MIP1a Matched Antibody Pair Kit
See all Macrophage Inflammatory Protein 1 alpha / CCL3 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
0.04 pg/ml -
Range
0.14 pg/ml - 100 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human MIP1a Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human MIP1a.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab214569. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human MIP1a Capture Antibody 2 x 50µg 1 x 50µg Human MIP1a Detector Antibody 2 x 12.5µg 1 x 12.5µg Human MIP1a Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsN-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T-cells. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C C motif chemokine 3
- CCL 3
- CCL3
see all -
Database links
- Entrez Gene: 6348 Human
- Omim: 182283 Human
- SwissProt: P10147 Human
- Unigene: 514107 Human
Images
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Human MIP1a standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human MIP1a Matched Antibody Pair Kit (ab216069)To learn more about the advantages of recombinant antibodies see here.
