Human IL-36 alpha ELISA (IL-1F6) (ab267641)
Key features and details
- Sensitivity: 0.8 ng/ml
- Range: 0.819 ng/ml - 200 ng/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-36 alpha ELISA (IL-1F6) -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.8 ng/ml -
Range
0.819 ng/ml - 200 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 73.74 69% - 79% Plasma 72.6 68% - 79% Cell culture media 89.23 82% - 99% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human IL-36 alpha ELISA Kit is designed for the quantitative determination of IL-36 alpha in cell culture supernatants, plasma and serum samples.
Δ Note: Human IL-1F6 concentration is low in normal serum/plasma, and it may not be detected in this assay.
Δ Note: Collect plasma using EDTA and citrate as anticoagulants; Heparin is not recommended for use in this assay.
This assay employs an antibody specific for human IL-36 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-36 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-36 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-36 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Anti-Human IL-36 alpha ELISA coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Human IL-36 alpha ELISA Detection Antibody 2 vials Human IL-36 alpha ELISA Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Tissue specificity
Expressed in immune system and fetal brain, but not in other tissues tested or in multiple hematopoietic cell lines. -
Sequence similarities
Belongs to the IL-1 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- FIL1
- FIL1 epsilon
- FIL1(EPSILON)
see all -
Database links
- Entrez Gene: 27179 Human
- Omim: 605509 Human
- SwissProt: Q9UHA7 Human
- Unigene: 278910 Human
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.