Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric - Red) (ab219925)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell Lysate
- Sensitivity: 0.1 mU/ml
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 200 tests Assay Buffer 1 x 20ml DMSO 1 x 200µl H2O2 (3%) 1 x 100µl Myeloperoxidase Standard (10 mU) 1 vial Red Dye (light-sensitive) 1 vial -
Research areas
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Function
Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. -
Involvement in disease
Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis. -
Sequence similarities
Belongs to the peroxidase family. XPO subfamily. -
Cellular localization
Lysosome. - Information by UniProt
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Alternative names
- 84 kDa myeloperoxidase
- 89 kDa myeloperoxidase
- EC 1.11.1.7
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Images
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Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric - Red) (ab219925)
Typical Myeloperoxidase (MPO) dose response curve. Absorbance was measured on a 96-well black solid plate using a Gemini fluoresence microplate reader (Molecular Devices). As low as 0.1 mU/mL Myeloperoxidase (MPO) activity can be detected after 60 minutes incubation.
