Myeloperoxidase (MPO) Inhibitor Screening Assay Kit (ab133080)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
Overview
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Product name
Myeloperoxidase (MPO) Inhibitor Screening Assay Kit
See all Myeloperoxidase kits -
Detection method
Fluorescent -
Precision
Intra-assay Sample n Mean SD CV% Chlorination 16 3.7% Peroxidation 16 2.8% Inter-assay Sample n Mean SD CV% Chlorination 16 3.6% Peroxidation 16 3.1% -
Assay type
Quantitative -
Species reactivity
Reacts with: Human
Predicted to work with: Mammals
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Product overview
Abcam’s Myeloperoxidase (MPO) Inhibitor Screening Assay (ab133080) provides convenient fluorescence-based methods for screening inhibitors to both the chlorination and peroxidation activities of MPO. The chlorination assay utilizes the non-fluorescent 2-[6-(4-aminophenoxy)-3-oxo-3H-xanthen-9-yl]-benzoic acid (APF), which is selectively cleaved by hypochlorite (-OCl) to yield the highly fluorescent compound fluorescein. Fluorescein fluorescence is analyzed with an excitation wavelength of 480-490 nm and an emission wavelength of 515-520 nm. The peroxidation assay utilizes the peroxidase component of MPO, where a single two electron oxidation of native enzyme (MPO) to compound I (MPO-I) is followed by two successive one electron reductions back to native enzyme by compound II (MPO-II). The reaction between hydrogen peroxide and ADHP (10-acetyl-3,7-dihydroxyphenoxazine) produces the highly fluorescent compound resorufin. Resorufin fluorescence is analyzed with an excitation wavelength of 530-540 nm and an emission wavelength of 585-595 nm.
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Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components 2 x 96 tests 96-Well Plate (black) 2 units 96-Well Plate Cover 2 units ADHP Assay Reagent 2 vials MPO Assay Buffer 1 vial MPO Chlorination Substrate 1 vial MPO DMSO 1 x 1ml MPO Hydrogen Peroxide 1 vial MPO Inhibitor 1 vial Myeloperoxidase Assay Reagent 1 vial -
Research areas
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Function
Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. -
Involvement in disease
Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis. -
Sequence similarities
Belongs to the peroxidase family. XPO subfamily. -
Cellular localization
Lysosome. - Information by UniProt
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Alternative names
- 84 kDa myeloperoxidase
- 89 kDa myeloperoxidase
- EC 1.11.1.7
see all -
Database links
- Entrez Gene: 4353 Human
- Omim: 606989 Human
- SwissProt: P05164 Human
- Unigene: 458272 Human
Images
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Functional Studies - Myeloperoxidase (MPO) Inhibitor Screening Assay Kit (ab133080)Inhibition of MPO peroxidation activity by 4-aminobenzhydrazide (IC50 = 0.4 µM).
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Functional Studies - Myeloperoxidase (MPO) Inhibitor Screening Assay Kit (ab133080)Inhibition of MPO chlorination activity by 4-aminobenzhydrazide (IC50 = 5 nM).
